PMID- 22718504
OWN - NLM
STAT- MEDLINE
DCOM- 20121214
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 9
DP  - 2012 Sep
TI  - Evaluation of oxidative stress markers in pathogenesis of diabetic neuropathy.
PG  - 8669-78
LID - 10.1007/s11033-012-1722-9 [doi]
AB  - Experimental evidences suggest that hyperglycaemia-induced overproduction of 
      reactive oxygen species and subsequent damage to proteins, lipids and DNA may 
      play a key role in the development of distal symmetric polyneuropathy (DSPN)-the 
      most common complication of diabetes mellitus. The study population consisted of 
      51 individuals aged 52-82 years classified into 3 groups: 16 patients diagnosed 
      with type 2 diabetes mellitus (T2DM) with DSPN, 16 T2DM patients without DSPN and 
      19 control subjects without diabetes and neuropathy. The study was conducted to 
      determine the activity of antioxidant enzymes: catalase (CAT), superoxide 
      dismutase (SOD), glutathione peroxidase (GPX) and total antioxidant status (TAS) 
      in the examined groups. An alkaline comet assay was used to determine the extent 
      of DNA damage of oxidized purines as glicosylo-formamidoglicosylase (Fpg) sites, 
      and oxidized pyrimidines as endonuclease III (Nth) sites. A significant decrease 
      of SOD (P < 0.05), GPX (P < 0.05) and nonsignificant decrease of CAT (P > 0.05), 
      and TAS status (P > 0.05) were seen in T2DM patients with neuropathy compared to 
      T2DM patients as well as controls. T2DM patients with or without neuropathy 
      revealed significantly lower (P < 0.05) plasma concentration of nitrous oxide 
      compared to the control subjects. Endogenous level of oxidative DNA damage in 
      T2DM patients with DSPN was significantly higher compared both to the controls 
      and T2DM patients without DSPN (P < 0.001). Moreover, lymphocytes isolated from 
      T2DM patients with DSPN were more susceptible to oxidative DNA lesions induced by 
      hydrogen peroxide than from T2DM patients without DSPN (P < 0.001). Our results 
      confirm hypothesis that oxidative stress may play a substantial role in the 
      development and progression of diabetic distal symmetric polyneuropathy.
FAU - Kasznicki, Jacek
AU  - Kasznicki J
AD  - Department of Internal Medicine, Diabetology and Clinical Pharmacology, Medical 
      University of Lodz, ul. Parzeczewska 35, 95-100 Zgierz, Poland. 
      jacek.kasznicki@umed.lodz.pl
FAU - Kosmalski, Marcin
AU  - Kosmalski M
FAU - Sliwinska, Agnieszka
AU  - Sliwinska A
FAU - Mrowicka, Malgorzata
AU  - Mrowicka M
FAU - Stanczyk, Malgorzata
AU  - Stanczyk M
FAU - Majsterek, Ireneusz
AU  - Majsterek I
FAU - Drzewoski, Jozef
AU  - Drzewoski J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120621
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antioxidants/metabolism
MH  - *Biomarkers
MH  - DNA Damage
MH  - Diabetic Neuropathies/*genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Oxidative Stress
MH  - Superoxide Dismutase/metabolism
PMC - PMC3404273
EDAT- 2012/06/22 06:00
MHDA- 2012/12/15 06:00
PMCR- 2012/06/21
CRDT- 2012/06/22 06:00
PHST- 2012/02/02 00:00 [received]
PHST- 2012/06/06 00:00 [accepted]
PHST- 2012/06/22 06:00 [entrez]
PHST- 2012/06/22 06:00 [pubmed]
PHST- 2012/12/15 06:00 [medline]
PHST- 2012/06/21 00:00 [pmc-release]
AID - 1722 [pii]
AID - 10.1007/s11033-012-1722-9 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Sep;39(9):8669-78. doi: 10.1007/s11033-012-1722-9. Epub 2012 
      Jun 21.