PMID- 22720952
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20191210
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 95
IP  - 7
DP  - 2012 Jul
TI  - Effects of dietary carbohydrates on rumen epithelial metabolism of nonlactating 
      heifers.
PG  - 3977-86
LID - 10.3168/jds.2011-5089 [doi]
AB  - Ruminal wall metabolism was studied in nonlactating heifers by altering the 
      carbohydrate (CHO) digestion site between rumen and intestine. The CHO digestion 
      site was estimated from in situ and total-tract digestibility of control (CONT) 
      diets and diets supplemented with corn (CRN), barley (BARL), or soy hulls (SOYH). 
      Ruminal epithelial metabolism regulating gene expression, morphology, and 
      nutrient delivery was assessed from a combination of rumen volatile fatty acid 
      (VFA) concentration, biopsies for papilla morphology, and expression of putative 
      metabolic regulatory genes encoding enzymes that facilitate VFA utilization. 
      Digestible dry matter and CHO intake were 25 and 45% higher, respectively, in the 
      supplemented diets than in CONT diets. Fiber supplementation increased the 
      intestinal and decreased ruminal CHO digestion. Ruminal nonfiber CHO 
      digestibility was 10% lower in CRN than with the high rumen-degradable 
      supplement. The CONT heifers had lowest total ruminal VFA and highest acetate 
      concentration relative to the other treatments. Total VFA concentration in BARL 
      and CRN diets tended to be higher than in SOYH. The SOYH diet tended to reduce 
      papilla dimension relative to CRN and BARL. The CRN diet tended to increase 
      papilla surface area relative to BARL and SOYH. Gene expression of 
      propionyl-coenzyme A carboxylase was higher in CRN and BARL than in SOYH diets, 
      and tended to be higher in CRN than in BARL and SOYH diets. Lactate dehydrogenase 
      and butyryl coenzyme A synthase gene transcripts tended to be higher in CONT than 
      in the supplemented treatments. Thus, rumen epithelial expression of genes 
      involved in VFA metabolism and ruminal wall-structure development are influenced 
      by other regulatory mechanism that is not directly affected by local signals. The 
      in situ methods used are a useful tool for differentiating ruminal from 
      extraruminal nutrient supply.
CI  - Copyright (c) 2012 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Argov-Argaman, N
AU  - Argov-Argaman N
AD  - The Department of Animal Science, The Robert H. Smith Faculty of Agriculture, 
      Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel. 
      argov@agri.huji.ac.il
FAU - Eshel, O
AU  - Eshel O
FAU - Moallem, U
AU  - Moallem U
FAU - Lehrer, H
AU  - Lehrer H
FAU - Uni, Z
AU  - Uni Z
FAU - Arieli, A
AU  - Arieli A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Fatty Acids, Volatile)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 6.2.1.- (Coenzyme A Ligases)
RN  - EC 7.2.4.3 (Methylmalonyl-CoA Decarboxylase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Coenzyme A Ligases/biosynthesis
MH  - Diet/veterinary
MH  - Dietary Carbohydrates/*pharmacology
MH  - Dietary Supplements
MH  - Epithelium/chemistry/drug effects/metabolism
MH  - Fatty Acids, Volatile/analysis
MH  - Female
MH  - Hordeum/metabolism
MH  - L-Lactate Dehydrogenase/biosynthesis
MH  - Methylmalonyl-CoA Decarboxylase/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Rumen/chemistry/drug effects/*metabolism
MH  - Zea mays/metabolism
EDAT- 2012/06/23 06:00
MHDA- 2012/11/06 06:00
CRDT- 2012/06/23 06:00
PHST- 2011/10/26 00:00 [received]
PHST- 2012/02/26 00:00 [accepted]
PHST- 2012/06/23 06:00 [entrez]
PHST- 2012/06/23 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
AID - S0022-0302(12)00375-X [pii]
AID - 10.3168/jds.2011-5089 [doi]
PST - ppublish
SO  - J Dairy Sci. 2012 Jul;95(7):3977-86. doi: 10.3168/jds.2011-5089.