PMID- 22722650
OWN - NLM
STAT- MEDLINE
DCOM- 20121224
LR  - 20220408
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 159
IP  - 3
DP  - 2012
TI  - Varying allergen composition and content affects the in vivo allergenic activity 
      of commercial Dermatophagoides pteronyssinus extracts.
PG  - 253-62
LID - 10.1159/000337654 [doi]
AB  - BACKGROUND: Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still 
      based on natural allergen extracts. The aim of this study was to analyze 
      commercially available Dermatophagoides pteronyssinus extracts from different 
      manufacturers regarding allergen composition and content and whether variations 
      may affect their allergenic activity. METHODS: Antibodies specific for several D. 
      pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze 
      extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were 
      used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 
      45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) 
      reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 
      and 21) by dot blot. RESULTS: Only Der p 1 and Der p 2 were detected in all 
      extracts but their concentrations and ratios showed high variability (Der p 1: 
      6.0-40.8 microg ml(-1); Der p 2: 1.7-45.0 microg ml(-1)). At least 1 out of 4 allergens 
      (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. 
      Mite-allergic subjects showed different IgE reactivity profiles to the individual 
      mite allergens, the extracts showed different allergenic activity in skin-prick 
      tests and false-negative results. CONCLUSIONS: Commercially available D. 
      pteronyssinus extracts lack important allergens, show great variability regarding 
      allergen composition and content and some gave false-negative diagnostic test 
      results in certain patients.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Casset, Anne
AU  - Casset A
AD  - Division of Immunopathology, Department of Pathophysiology and Allergy Research, 
      Center for Pathophysiology, Infectiology and Immunology, Medical University of 
      Vienna, Vienna, Austria.
FAU - Mari, Adriano
AU  - Mari A
FAU - Purohit, Ashok
AU  - Purohit A
FAU - Resch, Yvonne
AU  - Resch Y
FAU - Weghofer, Margit
AU  - Weghofer M
FAU - Ferrara, Rosetta
AU  - Ferrara R
FAU - Thomas, Wayne R
AU  - Thomas WR
FAU - Alessandri, Claudia
AU  - Alessandri C
FAU - Chen, Kuan-Wei
AU  - Chen KW
FAU - de Blay, Frederic
AU  - de Blay F
FAU - Valenta, Rudolf
AU  - Valenta R
FAU - Vrtala, Susanne
AU  - Vrtala S
LA  - eng
GR  - F 4602/FWF_/Austrian Science Fund FWF/Austria
GR  - F 4605/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120621
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Complex Mixtures)
RN  - 0 (Dermatophagoides pteronyssinus antigen p 2)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
SB  - IM
CIN - Int Arch Allergy Immunol. 2013;161(3):285-6. PMID: 23548609
CIN - Int Arch Allergy Immunol. 2013;161(3):287-8. PMID: 23548663
MH  - Adult
MH  - Allergens/chemistry/*immunology
MH  - Animals
MH  - Antibodies/blood/immunology
MH  - Antibody Diversity
MH  - Antigens, Dermatophagoides/blood/*immunology
MH  - Arthropod Proteins/blood/*immunology
MH  - Complex Mixtures/chemistry/immunology
MH  - Cysteine Endopeptidases/blood/*immunology
MH  - Dermatitis, Contact/blood/diagnosis/*immunology
MH  - Dermatophagoides pteronyssinus/chemistry/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - False Negative Reactions
MH  - Humans
MH  - Immunoglobulin E/blood/immunology
MH  - Skin Tests
PMC - PMC4594775
MID - EMS65147
OID - NLM: EMS65147
EDAT- 2012/06/23 06:00
MHDA- 2012/12/25 06:00
PMCR- 2015/10/06
CRDT- 2012/06/23 06:00
PHST- 2011/03/16 00:00 [received]
PHST- 2012/02/22 00:00 [accepted]
PHST- 2012/06/23 06:00 [entrez]
PHST- 2012/06/23 06:00 [pubmed]
PHST- 2012/12/25 06:00 [medline]
PHST- 2015/10/06 00:00 [pmc-release]
AID - 000337654 [pii]
AID - 10.1159/000337654 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2012;159(3):253-62. doi: 10.1159/000337654. Epub 2012 
      Jun 21.