PMID- 22726556
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20131121
IS  - 1467-7652 (Electronic)
IS  - 1467-7644 (Linking)
VI  - 10
IP  - 8
DP  - 2012 Oct
TI  - Tuber-specific silencing of asparagine synthetase-1 reduces the 
      acrylamide-forming potential of potatoes grown in the field without affecting 
      tuber shape and yield.
PG  - 913-24
LID - 10.1111/j.1467-7652.2012.00720.x [doi]
AB  - Simultaneous silencing of asparagine synthetase (Ast)-1 and -2 limits asparagine 
      (ASN) formation and, consequently, reduces the acrylamide-forming potential of 
      tubers. The phenotype of silenced lines appears normal in the greenhouse, but 
      field-grown tubers are small and cracked. Assessing the effects of silencing 
      StAst1 and StAst2 individually, we found that yield drag was mainly linked to 
      down-regulation of StAst2. Interestingly, tubers from untransformed scions 
      grafted onto intragenic StAst1/2-silenced rootstock contained almost the same low 
      ASN levels as those in the original silenced lines, indicating that ASN is mainly 
      formed in tubers rather than being transported from leaves. This conclusion was 
      further supported by the finding that overexpression of StAst2 caused ASN to 
      accumulate in leaves but not tubers. Thus, ASN does not appear to be the main 
      form of organic nitrogen transported from leaves to tubers. Because reduced ASN 
      levels coincided with increased levels of glutamine, it appears likely that this 
      alternative amide amino acid is mobilized to tubers, where it is converted into 
      ASN by StAst1. Indeed, tuber-specific silencing of StAst1, but not of StAst2, was 
      sufficient to substantially lower ASN formation in tubers. Extensive field 
      studies demonstrated that the reduced acrylamide-forming potential achieved by 
      tuber-specific StAst1 silencing did not affect the yield or quality of 
      field-harvested tubers.
CI  - (c) 2012 The Authors. Plant Biotechnology Journal (c) 2012 Society for Experimental 
      Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
FAU - Chawla, Rekha
AU  - Chawla R
AD  - Simplot Plant Sciences, J. R. Simplot Company, Boise, ID, USA.
FAU - Shakya, Roshani
AU  - Shakya R
FAU - Rommens, Caius M
AU  - Rommens CM
LA  - eng
PT  - Journal Article
DEP - 20120622
PL  - England
TA  - Plant Biotechnol J
JT  - Plant biotechnology journal
JID - 101201889
RN  - 20R035KLCI (Acrylamide)
RN  - 7006-34-0 (Asparagine)
RN  - EC 6.3.1.1 (Aspartate-Ammonia Ligase)
SB  - IM
MH  - Acrylamide/*metabolism
MH  - Asparagine/genetics/metabolism
MH  - Aspartate-Ammonia Ligase/genetics/*metabolism
MH  - Down-Regulation
MH  - Gene Expression Regulation, Plant
MH  - *Gene Silencing
MH  - Genes, Plant
MH  - Genetic Engineering
MH  - Phenotype
MH  - Plant Tubers/*anatomy & histology/*enzymology
MH  - Plants, Genetically Modified
MH  - Solanum tuberosum/*chemistry/*genetics
EDAT- 2012/06/26 06:00
MHDA- 2013/03/08 06:00
CRDT- 2012/06/26 06:00
PHST- 2012/06/26 06:00 [entrez]
PHST- 2012/06/26 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
AID - 10.1111/j.1467-7652.2012.00720.x [doi]
PST - ppublish
SO  - Plant Biotechnol J. 2012 Oct;10(8):913-24. doi: 10.1111/j.1467-7652.2012.00720.x. 
      Epub 2012 Jun 22.