PMID- 22726612
OWN - NLM
STAT- MEDLINE
DCOM- 20130104
LR  - 20120817
IS  - 1472-4669 (Electronic)
IS  - 1472-4669 (Linking)
VI  - 10
IP  - 5
DP  - 2012 Sep
TI  - Nitrospira-dominated biofilm within a thermal artesian spring: a case for 
      nitrification-driven primary production in a geothermal setting.
PG  - 457-66
LID - 10.1111/j.1472-4669.2012.00335.x [doi]
AB  - Water chemistry, energetic modeling, and molecular analyses were combined to 
      investigate the microbial ecology of a biofilm growing in a thermal artesian 
      spring within Hot Springs National Park, AR. This unique fresh water spring has a 
      low dissolved chemical load and is isolated from both light and direct 
      terrestrial carbon input - resulting in an oligotrophic ecosystem limited for 
      fixed carbon and electron donors. Evaluation of energy yields of lithotrophic 
      reactions putatively linked to autotrophy identified the aerobic oxidation of 
      methane, hydrogen, sulfide, ammonia, and nitrite as the most exergonic. Small 
      subunit (SSU) rRNA gene libraries from biofilm revealed a low-diversity microbial 
      assemblage populated by bacteria and archaea at a gene copy ratio of 45:1. 
      Members of the bacterial family 'Nitrospiraceae', known for their autotrophic 
      nitrite oxidation, dominated the bacterial SSU rRNA gene library (approximately 
      45%). Members of the Thaumarchaeota ThAOA/HWCGIII (>96%) and Thaumarchaeota Group 
      I.1b (2.5%), which both contain confirmed autotrophic ammonia oxidizers, 
      dominated the archaeal SSU rRNA library. Archaea appear to dominate among the 
      ammonia oxidizers, as only ammonia monooxygenase subunit A (amoA) genes belonging 
      to members of the Thaumarchaeota were detected. The geochemical, phylogenetic, 
      and genetic data support a model that describes a novel thermophilic biofilm 
      built largely by an autotrophic nitrifying microbial assemblage. This is also the 
      first observation of 'Nitrospiraceae' as the dominant organisms within a 
      geothermal environment.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Marks, C R
AU  - Marks CR
AD  - Department of Botany and Microbiology, University of Oklahoma, Norman, OK, USA.
FAU - Stevenson, B S
AU  - Stevenson BS
FAU - Rudd, S
AU  - Rudd S
FAU - Lawson, P A
AU  - Lawson PA
LA  - eng
SI  - GENBANK/JN807645
SI  - GENBANK/JN807646
SI  - GENBANK/JN807647
SI  - GENBANK/JN807648
SI  - GENBANK/JN807649
SI  - GENBANK/JN807650
SI  - GENBANK/JN807651
SI  - GENBANK/JN807652
SI  - GENBANK/JN807653
SI  - GENBANK/JN807654
SI  - GENBANK/JN807655
SI  - GENBANK/JN807656
SI  - GENBANK/JN807657
SI  - GENBANK/JN807658
SI  - GENBANK/JN807659
SI  - GENBANK/JN807660
SI  - GENBANK/JN807661
SI  - GENBANK/JN807662
SI  - GENBANK/JN807663
SI  - GENBANK/JN807664
SI  - GENBANK/JN807665
SI  - GENBANK/JN807666
SI  - GENBANK/JN807667
SI  - GENBANK/JN807668
SI  - GENBANK/JN807669
SI  - GENBANK/JN807670
SI  - GENBANK/JN807671
SI  - GENBANK/JN807672
SI  - GENBANK/JN807673
SI  - GENBANK/JN807674
SI  - GENBANK/JN807675
SI  - GENBANK/JN807676
SI  - GENBANK/JN807677
SI  - GENBANK/JN807678
SI  - GENBANK/JN807679
SI  - GENBANK/JN807680
SI  - GENBANK/JN807681
SI  - GENBANK/JN807682
SI  - GENBANK/JN807683
SI  - GENBANK/JN807684
SI  - GENBANK/JN807685
SI  - GENBANK/JN807686
SI  - GENBANK/JN807687
SI  - GENBANK/JN807688
SI  - GENBANK/JN807689
SI  - GENBANK/JN807690
SI  - GENBANK/JN807691
SI  - GENBANK/JN807692
SI  - GENBANK/JN807693
SI  - GENBANK/JN807694
SI  - GENBANK/JN807695
SI  - GENBANK/JN807696
SI  - GENBANK/JN807697
SI  - GENBANK/JN807698
SI  - GENBANK/JN807699
SI  - GENBANK/JN807700
SI  - GENBANK/JN807701
SI  - GENBANK/JN807702
SI  - GENBANK/JN807703
SI  - GENBANK/JN807704
SI  - GENBANK/JN807705
SI  - GENBANK/JN807706
SI  - GENBANK/JN807707
SI  - GENBANK/JN807708
SI  - GENBANK/JN807709
SI  - GENBANK/JN807710
SI  - GENBANK/JN807711
SI  - GENBANK/JN807712
SI  - GENBANK/JN807713
SI  - GENBANK/JN807714
SI  - GENBANK/JN807715
SI  - GENBANK/JN807716
SI  - GENBANK/JN807717
SI  - GENBANK/JN807718
SI  - GENBANK/JN807719
SI  - GENBANK/JN807720
SI  - GENBANK/JN807721
SI  - GENBANK/JN807722
SI  - GENBANK/JN807723
SI  - GENBANK/JN807724
SI  - GENBANK/JN807725
SI  - GENBANK/JN807726
SI  - GENBANK/JN807727
SI  - GENBANK/JN807728
SI  - GENBANK/JN807729
SI  - GENBANK/JN807730
SI  - GENBANK/JN807731
SI  - GENBANK/JN807732
SI  - GENBANK/JN807733
SI  - GENBANK/JN807734
SI  - GENBANK/JN807735
SI  - GENBANK/JN807736
SI  - GENBANK/JN807737
SI  - GENBANK/JN807738
SI  - GENBANK/JN807739
SI  - GENBANK/JN807740
SI  - GENBANK/JN807741
SI  - GENBANK/JN807742
SI  - GENBANK/JN807743
SI  - GENBANK/JN807744
SI  - GENBANK/JN807745
SI  - GENBANK/JN807746
SI  - GENBANK/JN807747
SI  - GENBANK/JN807748
SI  - GENBANK/JN807749
SI  - GENBANK/JN807750
SI  - GENBANK/JN807751
SI  - GENBANK/JN807752
SI  - GENBANK/JN807753
SI  - GENBANK/JN807754
SI  - GENBANK/JN807755
SI  - GENBANK/JN807756
SI  - GENBANK/JN807757
SI  - GENBANK/JN807758
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120621
PL  - England
TA  - Geobiology
JT  - Geobiology
JID - 101185472
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Archaeal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Archaea/classification/*isolation & purification/metabolism
MH  - Arkansas
MH  - Bacteria/classification/*isolation & purification/metabolism
MH  - Biofilms/*growth & development
MH  - *Biota
MH  - Cluster Analysis
MH  - DNA, Archaeal/chemistry/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Genes, rRNA
MH  - Hot Springs/*microbiology
MH  - Molecular Sequence Data
MH  - *Nitrification
MH  - Oxidoreductases/genetics
MH  - Phylogeny
MH  - RNA, Archaeal/genetics
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
EDAT- 2012/06/26 06:00
MHDA- 2013/01/05 06:00
CRDT- 2012/06/26 06:00
PHST- 2011/11/03 00:00 [received]
PHST- 2012/05/15 00:00 [accepted]
PHST- 2012/06/26 06:00 [entrez]
PHST- 2012/06/26 06:00 [pubmed]
PHST- 2013/01/05 06:00 [medline]
AID - 10.1111/j.1472-4669.2012.00335.x [doi]
PST - ppublish
SO  - Geobiology. 2012 Sep;10(5):457-66. doi: 10.1111/j.1472-4669.2012.00335.x. Epub 
      2012 Jun 21.