PMID- 2272663
OWN - NLM
STAT- MEDLINE
DCOM- 19910222
LR  - 20190828
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 32
IP  - 6
DP  - 1990
TI  - Molecular analysis of the human MHC class I region using yeast artificial 
      chromosome clones.
PG  - 419-26
AB  - The cloning of large genomic fragments corresponding to the major 
      histocompatibility complex (MHC) class I region provides the necessary framework 
      for a better understanding of its organization and for the localization of new 
      genes involved in MHC-associated disease. Two human genomic libraries constructed 
      in yeast artificial chromosomes (YACs) have been prepared using complete Not I or 
      Mlu I digestion of source DNA. From these libraries three YAC clones with inserts 
      belonging to the MHC class I region have been isolated. They correspond to exact 
      copies of three genomic fragments of 210, 145, and 50 kilobases (kb), 
      respectively and have been precisely located in the restriction map of the 
      region. Detailed rare-cutter restriction maps of the inserts have been generated. 
      Within these clones we have demonstrated the presence of two class I genes, one 
      of which is HLA-E, and of at least three Hpa II tiny fragment (HTF) islands, 
      corresponding to three putative new transcribed sequences. End clones, which are 
      of particular interest in the extension and refinement of the regional map, have 
      been rescued by systematic subcloning of purified YACs.
FAU - Chimini, G
AU  - Chimini G
AD  - Centre d'Immunologie INSERM-CNRS de Marseille Luminy, France.
FAU - Boretto, J
AU  - Boretto J
FAU - Marguet, D
AU  - Marguet D
FAU - Lanau, F
AU  - Lanau F
FAU - Lauquin, G
AU  - Lauquin G
FAU - Pontarotti, P
AU  - Pontarotti P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Base Sequence
MH  - *Chromosomes, Fungal
MH  - Cloning, Molecular
MH  - Gene Library
MH  - *Genes, MHC Class I
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Yeasts/*genetics
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1007/BF00241636 [doi]
PST - ppublish
SO  - Immunogenetics. 1990;32(6):419-26. doi: 10.1007/BF00241636.