PMID- 22730763
OWN - NLM
STAT- MEDLINE
DCOM- 20120801
LR  - 20181201
IS  - 0016-6758 (Print)
IS  - 0016-6758 (Linking)
VI  - 48
IP  - 4
DP  - 2012 Apr
TI  - [Selective staining of pericentromeric heterochromatin regions in chromosomes of 
      spontaneously dividing cells with the use of the acridine orange fluorochrome].
PG  - 451-6
AB  - A novel phenomenon of unusual selective acridine orange (AO) staining 
      ofpericentromeric heterochromatin regions (HRs) in chromosomal preparations from 
      tissue with known spontaneous mitotic activity (chorionic villi, placenta, 
      embryonic tissues, bone marrow, and testes), as well as embryonic stem cells, is 
      described. Staining with 0.01% AO in a citric-phosphate (pH 5.5) or sodium 
      phosphate (pH 7.0) buffer solution allows the HRs of human chromosomes (1q12, 
      9q12, 13p11.2, 14p11.2, 15p11.2, 16q11.2, 21p11.2, 22p11.2, and Yq12) and 
      pericentromeric HRs of mouse chromosomes to be reliably detected by the red 
      fluorescence of AO. This method of AO staining does not require any pretreatment. 
      Explanations for metachromatic AO staining of polymorphic pericentromeric HRs in 
      chromosomes of spontaneously dividing cells are suggested. A high reproducibility 
      of the specific AO staining makes it possible to suggest its use as a reliable 
      quick method for detection of polymorphic HRs of human chromosomes in cytogenetic 
      prenatal diagnosis and oncohematology.
FAU - Kuznetzova, T V
AU  - Kuznetzova TV
FAU - Trofimova, I L
AU  - Trofimova IL
FAU - Liapunov, M S
AU  - Liapunov MS
FAU - Evdokimenko, E V
AU  - Evdokimenko EV
FAU - Baranov, V S
AU  - Baranov VS
LA  - rus
PT  - Journal Article
PL  - Russia (Federation)
TA  - Genetika
JT  - Genetika
JID - 0047354
RN  - 0 (Heterochromatin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Division/genetics
MH  - Chorion/cytology
MH  - Chromosomes, Human/*genetics
MH  - Female
MH  - *Heterochromatin/genetics
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology
MH  - Metaphase/genetics
MH  - Mice
MH  - *Polymorphism, Genetic
MH  - Pregnancy
MH  - Staining and Labeling/methods
EDAT- 2012/06/27 06:00
MHDA- 2012/08/02 06:00
CRDT- 2012/06/27 06:00
PHST- 2012/06/27 06:00 [entrez]
PHST- 2012/06/27 06:00 [pubmed]
PHST- 2012/08/02 06:00 [medline]
PST - ppublish
SO  - Genetika. 2012 Apr;48(4):451-6.