PMID- 22730768 OWN - NLM STAT- MEDLINE DCOM- 20120801 LR - 20120626 IS - 0016-6758 (Print) IS - 0016-6758 (Linking) VI - 48 IP - 4 DP - 2012 Apr TI - [Construction and study of leaf rust-resistant common wheat lines with translocations of Aegilops speltoides Tausch]. PG - 488-94 AB - Genotyping was performed for the leaf rust-resistant line 73/00i (Triticum aestivum x Aegilops speltoides). Fluorescence in situ hybridization (FISH) with probes Spelt1 and pSc119.2 in combination with microsatellite analysis were used to determine the locations and sizes of the Ae. speltoides genetic fragments integrated into the line genome. Translocations were identified in the long arms of chromosomes 5B and 6B and in the short arm of chromosome 1B. The Spelt1 and pSc119.2 molecular cytological markers made it possible to rapidly establish lines with single translocation in the long arms of chromosomes 5B and 6B. The line carrying the T5BS x 5BL-5SL translocation was highly resistant to leaf rust, and the lines carrying the T6BS x 6BL-6SL translocation displayed moderate resistance. The translocations differed in chromosomal location from known leaf resistance genes transferred into common wheat from Ae. speltoides. Hence, it was assumed that new genes were introduced into the common wheat genome from Ae. speltoides. The locus that determined high resistance to leaf rust and was transferred into the common wheat genome from the long arm of Ae. speltoides chromosome 5S by the T5BS x 5BL-5SL translocation was preliminarily designated as LrAsp5. FAU - Adonina, I G AU - Adonina IG FAU - Petrash, N V AU - Petrash NV FAU - Timonova, E M AU - Timonova EM FAU - Khristov, Iu A AU - Khristov IuA FAU - Salina, E A AU - Salina EA LA - rus PT - English Abstract PT - Journal Article PL - Russia (Federation) TA - Genetika JT - Genetika JID - 0047354 SB - IM MH - Chimera/genetics MH - Cloning, Molecular MH - Disease Resistance/*genetics MH - Genotype MH - In Situ Hybridization, Fluorescence MH - Plant Diseases/*genetics MH - Plant Leaves/genetics MH - Poaceae/*genetics MH - Translocation, Genetic/*genetics MH - Triticum/*genetics EDAT- 2012/06/27 06:00 MHDA- 2012/08/02 06:00 CRDT- 2012/06/27 06:00 PHST- 2012/06/27 06:00 [entrez] PHST- 2012/06/27 06:00 [pubmed] PHST- 2012/08/02 06:00 [medline] PST - ppublish SO - Genetika. 2012 Apr;48(4):488-94.