PMID- 22731835
OWN - NLM
STAT- MEDLINE
DCOM- 20130502
LR  - 20151119
IS  - 1365-2230 (Electronic)
IS  - 0307-6938 (Linking)
VI  - 37
IP  - 8
DP  - 2012 Dec
TI  - Application of fluorescence in situ hybridization as a diagnostic tool in 
      melanocytic lesions, using paraffin wax-embedded tissues and imprint-cytology 
      specimens.
PG  - 838-43
LID - 10.1111/j.1365-2230.2012.04416.x [doi]
AB  - BACKGROUND: Accurate histopathological diagnosis of certain melanocytic skin 
      lesions as benign or malignant can be notoriously difficult. Recently, 
      four-colour fluorescence in situ hybridization (FISH) has emerged as an important 
      tool for classifying these lesions. AIM: To evaluate the sensitivity and 
      specificity of a melanoma FISH probe kit for accurate diagnosis of melanocytic 
      tumours, and to validate its use with imprint-cytology specimens from the cut 
      surface of tumours. METHODS: In total, 50 melanocytic skin lesions (31 malignant 
      melanomas, 10 benign melanocytic naevi, and 9 histologically challenging benign 
      melanocytic skin lesions) were evaluated. The samples comprise 47 tissue 
      specimens embedded in paraffin wax, and three imprint-cytology specimens from the 
      cut surface of melanomas. FISH was performed using four locus-specific identifier 
      probes [Ras responsive element binding protein (RREB)1, myeloblastosis viral 
      oncogene homologue (MYB), cyclin (CCN)D1 and centromere of chromosome (CEP)6], 
      and results were compared with the clinical long-term follow-up and 
      histopathological diagnosis data. RESULTS: The melanoma FISH probe distinguished 
      between naevi and melanomas with a sensitivity of 100% and a specificity of 
      94.1%. The most sensitive criterion was a gain in 6p25 (RREB1), seen in 100% of 
      cases, followed by CEP6-related MYB loss (48.1%), CCND1 gain (37%) and MYB gain 
      (22.2%). More than three-quarters (77.8%) of melanomas were positive for two or 
      more criteria. Positive FISH results were also obtained for the imprint-cytology 
      specimens. CONCLUSIONS: FISH is a valuable diagnostic tool for differentiating 
      between benign and malignant melanocytic lesions, providing a high degree of 
      sensitivity and specificity. The probes displayed exceptional discriminative 
      capacity in difficult or ambiguous lesions. To our knowledge, his is the first 
      reported use of imprint-cytology specimens for FISH diagnosis.
CI  - (c) The Author(s). CED (c) 2012 British Association of Dermatologists.
FAU - Abasolo, A
AU  - Abasolo A
AD  - Department of Pathology, Virgen Macarena University Hospital, Seville, Spain.
FAU - Vargas, M T
AU  - Vargas MT
FAU - Rios-Martin, J J
AU  - Rios-Martin JJ
FAU - Trigo, I
AU  - Trigo I
FAU - Arjona, A
AU  - Arjona A
FAU - Gonzalez-Campora, R
AU  - Gonzalez-Campora R
LA  - eng
PT  - Journal Article
DEP - 20120625
PL  - England
TA  - Clin Exp Dermatol
JT  - Clinical and experimental dermatology
JID - 7606847
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RREB1 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - Cytological Techniques/*methods
MH  - DNA Probes
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Nevus, Pigmented/*diagnosis
MH  - Paraffin Embedding
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*diagnosis
MH  - Transcription Factors
MH  - Young Adult
EDAT- 2012/06/27 06:00
MHDA- 2013/05/03 06:00
CRDT- 2012/06/27 06:00
PHST- 2012/06/27 06:00 [entrez]
PHST- 2012/06/27 06:00 [pubmed]
PHST- 2013/05/03 06:00 [medline]
AID - 10.1111/j.1365-2230.2012.04416.x [doi]
PST - ppublish
SO  - Clin Exp Dermatol. 2012 Dec;37(8):838-43. doi: 10.1111/j.1365-2230.2012.04416.x. 
      Epub 2012 Jun 25.