PMID- 22732094
OWN - NLM
STAT- MEDLINE
DCOM- 20130108
LR  - 20231213
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 73
IP  - 9
DP  - 2012 Sep
TI  - Full-length HLA-G1 and truncated HLA-G3 differentially increase HLA-E surface 
      localization.
PG  - 898-905
LID - 10.1016/j.humimm.2012.06.007 [doi]
AB  - Human leukocyte antigen (HLA)-E plays a role in immune tolerance induction and 
      its transport to the cell surface is limited and dependent on the availability of 
      HLA class I signal peptide. The role of HLA-G in regulating HLA-E surface 
      localization remains controversial. The aim of our study was to clarify whether 
      full-length and truncated HLA-G isoforms regulate HLA-E surface localization. 
      Using a retroviral expression system and flow cytometric analysis, we found that 
      surface HLA-E levels were significantly higher in HLA-G1 (34.1+/-4.4%, p<0.005) and 
      -G3 (15.3+/-1.8%, p<0.04) versus empty vector (9.0+/-1.0%) transductants. 
      Biotinylation and Western blot studies revealed HLA-E surface protein was 
      increased by 4.5- and 1.3-fold in HLA-G1 and -G3 versus empty vector 
      transductants. Although no significant differences in transcript and protein 
      levels were detected between HLA-G1 and -G3 transductants, surface levels of 
      HLA-G1 were 2.5-fold higher than HLA-G3 by flow cytometric analysis and Western 
      blotting. Taken together, our data demonstrate that full-length HLA-G1 and 
      truncated -G3 differentially increase HLA-E surface localization.
CI  - Copyright (c) 2012 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Teklemariam, Takele
AU  - Teklemariam T
AD  - Escape Therapeutics, Inc., San Jose, CA, United States.
FAU - Zhao, Longmei
AU  - Zhao L
FAU - Hantash, Basil M
AU  - Hantash BM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120622
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Cell Membrane/*metabolism
MH  - Gene Expression Regulation
MH  - HLA-G Antigens/*genetics/*metabolism
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - K562 Cells
MH  - Protein Transport
MH  - HLA-E Antigens
EDAT- 2012/06/27 06:00
MHDA- 2013/01/09 06:00
CRDT- 2012/06/27 06:00
PHST- 2012/01/26 00:00 [received]
PHST- 2012/05/28 00:00 [revised]
PHST- 2012/06/13 00:00 [accepted]
PHST- 2012/06/27 06:00 [entrez]
PHST- 2012/06/27 06:00 [pubmed]
PHST- 2013/01/09 06:00 [medline]
AID - S0198-8859(12)00149-8 [pii]
AID - 10.1016/j.humimm.2012.06.007 [doi]
PST - ppublish
SO  - Hum Immunol. 2012 Sep;73(9):898-905. doi: 10.1016/j.humimm.2012.06.007. Epub 2012 
      Jun 22.