PMID- 22732188
OWN - NLM
STAT- MEDLINE
DCOM- 20130808
LR  - 20220317
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 21
IP  - 7
DP  - 2012
TI  - Human umbilical cord mesenchymal stem cells support nontumorigenic expansion of 
      human embryonic stem cells.
PG  - 1515-27
AB  - The expansion of pluripotent human embryonic stem cells (hESCs) requires a 
      culture on feeder layers of mouse embryonic fibroblasts (MEFs). The culture model 
      often causes immunogenic contaminations such as xenocarbohydrate, and inevitably 
      forms teratoma in vivo. This study tested human umbilical cord-derived 
      mesenchymal stem cells (HUCMSCs) as the feeder for hESCs. Wharton's jelly-derived 
      HUCMSCs showed characteristics of MSCs and were easily maintained in a culture 
      for over 20 passages. Under the mitomycin-inhibited HUCMSC feeder, hESCs 
      maintained the features of embryonic stem cells (pluripotency and maintenance of 
      normal karyotypes) after a prolonged culture of more than 20 passages. Notably, 
      in extensive trials, no teratoma was formed in xenograft in NOD/SCID mice, but 
      subsequent resumption of teratoma formation was noted upon transient coculturing 
      with MEFs. Interestingly, among the four pluripotency-conferring genes, MYC and 
      OCT4 were found to be downregulated in hESCs cocultured with HUCMSCs. Results of 
      this study supported a nontumorigenic sustained culture of hESCs and did not form 
      teratoma in vivo.
FAU - Ding, Dah-Ching
AU  - Ding DC
AD  - Department of Obstetrics and Gynecology, Buddhist Tzu Chi General Hospital, Tzu 
      Chi University, Hualien, Taiwan.
FAU - Shyu, Woei-Cherng
AU  - Shyu WC
FAU - Lin, Shinn-Zong
AU  - Lin SZ
FAU - Liu, Hwan-Wun
AU  - Liu HW
FAU - Chiou, Shih-Hwa
AU  - Chiou SH
FAU - Chu, Tang-Yuan
AU  - Chu TY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Down-Regulation
MH  - Embryonic Stem Cells/*cytology/metabolism/transplantation
MH  - Feeder Cells
MH  - Humans
MH  - Karyotyping
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Octamer Transcription Factor-3/metabolism
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Teratoma/pathology
MH  - Transplantation, Heterologous
MH  - Wharton Jelly/*cytology
EDAT- 2012/06/27 06:00
MHDA- 2013/08/09 06:00
CRDT- 2012/06/27 06:00
PHST- 2012/06/27 06:00 [entrez]
PHST- 2012/06/27 06:00 [pubmed]
PHST- 2013/08/09 06:00 [medline]
AID - ct0242ding [pii]
AID - 10.3727/096368912X647199 [doi]
PST - ppublish
SO  - Cell Transplant. 2012;21(7):1515-27. doi: 10.3727/096368912X647199.