PMID- 22734465
OWN - NLM
STAT- MEDLINE
DCOM- 20130604
LR  - 20211021
IS  - 1365-2982 (Electronic)
IS  - 1350-1925 (Print)
IS  - 1350-1925 (Linking)
VI  - 24
IP  - 10
DP  - 2012 Oct
TI  - Platelet-activating factor and distinct chemokines are elevated in mucosal 
      biopsies of erosive compared with non-erosive reflux disease patients and 
      controls.
PG  - 943-e463
LID - 10.1111/j.1365-2982.2012.01963.x [doi]
AB  - BACKGROUND: A distinction between symptomatic non-erosive reflux disease (NERD) 
      and erosive esophagitis (EE) patients is supported by the presence of 
      inflammatory response in the mucosa of EE patients, leading to a damage of 
      mucosal integrity. To explore the underlying mechanism of this difference, we 
      assessed inflammatory mediators in mucosal biopsies from EE and NERD patients and 
      compared them with controls. METHODS: Nineteen NERD patients, 15 EE patients, and 
      16 healthy subjects underwent endoscopy after a 3-week washout from PPI or H(2) 
      antagonists. Biopsies obtained from the distal esophagus were examined by 
      quantitative real-time polymerase chain reaction (qPCR) and multiplex 
      enzyme-linked immunosorbent assay for selected chemokines and lyso-PAF 
      acetyltransferase (LysoPAF-AT), the enzyme responsible for production of 
      platelet-activating factor (PAF). KEY RESULTS: Expression of LysoPAF-AT and 
      multiple chemokines was significantly increased in mucosal biopsies derived from 
      EE patients, when compared with NERD patients and healthy controls. Upregulated 
      chemokines included interleukin 8, eotaxin-1, -2, and -3, macrophage inflammatory 
      protein-1alpha (MIP-1alpha), and monocyte chemoattractant protein-1 (MCP-1). LysoPAF-AT 
      and the chemokine profile in NERD patients were comparable with healthy controls. 
      CONCLUSIONS & INFERENCES: Levels of selected cytokines and Lyso-PAF AT were 
      significantly higher in the esophageal mucosa of EE patients compared with NERD 
      and control patients. This difference may explain the distinct inflammatory 
      response occurring in EE patients' mucosa. In contrast, as no significant 
      differences existed between the levels of all mediators in NERD and control 
      subjects, an inflammatory response does not appear to play a major role in the 
      pathogenesis of the abnormalities found in NERD patients.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Altomare, A
AU  - Altomare A
AD  - Department of Digestive Disease, University Campus Bio-Medico, Via Alvaro del 
      Portillo, Rome, Italy. a.altomare@unicampus.it
FAU - Ma, J
AU  - Ma J
FAU - Guarino, M P L
AU  - Guarino MP
FAU - Cheng, L
AU  - Cheng L
FAU - Rieder, F
AU  - Rieder F
FAU - Ribolsi, M
AU  - Ribolsi M
FAU - Fiocchi, C
AU  - Fiocchi C
FAU - Biancani, P
AU  - Biancani P
FAU - Harnett, K
AU  - Harnett K
FAU - Cicala, M
AU  - Cicala M
LA  - eng
GR  - R01 DK057030/DK/NIDDK NIH HHS/United States
GR  - T32 DK083251/DK/NIDDK NIH HHS/United States
GR  - R01 57030/PHS HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120626
PL  - England
TA  - Neurogastroenterol Motil
JT  - Neurogastroenterology and motility
JID - 9432572
RN  - 0 (Chemokines)
RN  - 0 (Platelet Activating Factor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - Chemokines/analysis/*biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Esophagitis, Peptic/etiology/pathology
MH  - Female
MH  - Gastroesophageal Reflux/complications/*metabolism/*pathology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Platelet Activating Factor/analysis/*biosynthesis
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC3440538
MID - NIHMS383382
EDAT- 2012/06/28 06:00
MHDA- 2013/06/05 06:00
PMCR- 2013/10/01
CRDT- 2012/06/28 06:00
PHST- 2012/06/28 06:00 [entrez]
PHST- 2012/06/28 06:00 [pubmed]
PHST- 2013/06/05 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - 10.1111/j.1365-2982.2012.01963.x [doi]
PST - ppublish
SO  - Neurogastroenterol Motil. 2012 Oct;24(10):943-e463. doi: 
      10.1111/j.1365-2982.2012.01963.x. Epub 2012 Jun 26.