PMID- 22734669
OWN - NLM
STAT- MEDLINE
DCOM- 20121206
LR  - 20201209
IS  - 1365-2443 (Electronic)
IS  - 1356-9597 (Linking)
VI  - 17
IP  - 8
DP  - 2012 Aug
TI  - Dock3 regulates BDNF-TrkB signaling for neurite outgrowth by forming a ternary 
      complex with Elmo and RhoG.
PG  - 688-97
LID - 10.1111/j.1365-2443.2012.01616.x [doi]
AB  - Dock3, a new member of the guanine nucleotide exchange factor family, causes 
      cellular morphological changes by activating the small GTPase Rac1. 
      Overexpression of Dock3 in neural cells promotes neurite outgrowth through the 
      formation of a protein complex with Fyn and WAVE downstream of brain-derived 
      neurotrophic factor (BDNF) signaling. Here, we report a novel Dock3-mediated BDNF 
      pathway for neurite outgrowth. We show that Dock3 forms a complex with Elmo and 
      activated RhoG downstream of BDNF-TrkB signaling and induces neurite outgrowth 
      via Rac1 activation in PC12 cells. We also show the importance of Dock3 
      phosphorylation in Rac1 activation and show two key events that are necessary for 
      efficient Dock3 phosphorylation: membrane recruitment of Dock3 and interaction of 
      Dock3 with Elmo. These results suggest that Dock3 plays important roles 
      downstream of BDNF signaling in the central nervous system where it stimulates 
      actin polymerization by multiple pathways.
CI  - (c) 2012 The Authors Journal compilation (c) 2012 by the Molecular Biology Society of 
      Japan/Blackwell Publishing Ltd.
FAU - Namekata, Kazuhiko
AU  - Namekata K
AD  - Visual Research Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 
      156-8506, Japan.
FAU - Watanabe, Hayaki
AU  - Watanabe H
FAU - Guo, Xiaoli
AU  - Guo X
FAU - Kittaka, Daiji
AU  - Kittaka D
FAU - Kawamura, Kazuto
AU  - Kawamura K
FAU - Kimura, Atsuko
AU  - Kimura A
FAU - Harada, Chikako
AU  - Harada C
FAU - Harada, Takayuki
AU  - Harada T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120627
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Dock3 protein, mouse)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Rac1 protein, mouse)
RN  - 0 (Rhog protein, mouse)
RN  - 0 (Ternary Complex Factors)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - COS Cells
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Membrane/genetics/metabolism
MH  - Chlorocebus aethiops
MH  - Enzyme Activation
MH  - GTP Phosphohydrolases/genetics/metabolism
MH  - Guanine Nucleotide Exchange Factors
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurites/drug effects/*metabolism/physiology
MH  - Neuropeptides/genetics/metabolism
MH  - PC12 Cells
MH  - Phosphorylation
MH  - Protein Interaction Mapping
MH  - Protein Transport
MH  - Rats
MH  - Receptor, trkB/genetics/*metabolism
MH  - *Signal Transduction
MH  - Ternary Complex Factors/genetics/*metabolism
MH  - Transfection
MH  - rac GTP-Binding Proteins/genetics/metabolism
MH  - rac1 GTP-Binding Protein
MH  - rho GTP-Binding Proteins
EDAT- 2012/06/28 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/06/28 06:00
PHST- 2011/07/13 00:00 [received]
PHST- 2012/04/25 00:00 [accepted]
PHST- 2012/06/28 06:00 [entrez]
PHST- 2012/06/28 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1111/j.1365-2443.2012.01616.x [doi]
PST - ppublish
SO  - Genes Cells. 2012 Aug;17(8):688-97. doi: 10.1111/j.1365-2443.2012.01616.x. Epub 
      2012 Jun 27.