PMID- 22737243
OWN - NLM
STAT- MEDLINE
DCOM- 20121214
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 6
DP  - 2012
TI  - Electrical stimulation to conductive scaffold promotes axonal regeneration and 
      remyelination in a rat model of large nerve defect.
PG  - e39526
LID - 10.1371/journal.pone.0039526 [doi]
LID - e39526
AB  - BACKGROUND: Electrical stimulation (ES) has been shown to promote nerve 
      regeneration when it was applied to the proximal nerve stump. However, the 
      possible beneficial effect of establishing a local electrical environment between 
      a large nerve defect on nerve regeneration has not been reported in previous 
      studies. The present study attempted to establish a local electrical environment 
      between a large nerve defect, and examined its effect on nerve regeneration and 
      functional recovery. METHODOLOGY/FINDINGS: In the present study, a conductive 
      scaffold was constructed and used to bridge a 15 mm sciatic nerve defect in rats, 
      and intermittent ES (3 V, 20 Hz) was applied to the conductive scaffold to 
      establish an electrical environment at the site of nerve defect. Nerve 
      regeneration and functional recovery were examined after nerve injury repair and 
      ES. We found that axonal regeneration and remyelination of the regenerated axons 
      were significantly enhanced by ES which was applied to conductive scaffold. In 
      addition, both motor and sensory functional recovery was significantly improved 
      and muscle atrophy was partially reversed by ES localized at the conductive 
      scaffold. Further investigations showed that the expression of S-100, BDNF 
      (brain-derived neurotrophic factor), P0 and Par-3 was significantly up-regulated 
      by ES at the conductive scaffold. CONCLUSIONS/SIGNIFICANCE: Establishing an 
      electrical environment with ES localized at the conductive scaffold is capable of 
      accelerating nerve regeneration and promoting functional recovery in a 15 mm 
      nerve defect in rats. The findings provide new directions for exploring 
      regenerative approaches to achieve better functional recovery in the treatment of 
      large nerve defect.
FAU - Huang, Jinghui
AU  - Huang J
AD  - Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical 
      University, Xi'an, China.
FAU - Lu, Lei
AU  - Lu L
FAU - Zhang, Jianbin
AU  - Zhang J
FAU - Hu, Xueyu
AU  - Hu X
FAU - Zhang, Yongguang
AU  - Zhang Y
FAU - Liang, Wei
AU  - Liang W
FAU - Wu, Siyu
AU  - Wu S
FAU - Luo, Zhuojing
AU  - Luo Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120621
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carrier Proteins)
RN  - 0 (Myelin P0 Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Pard3 protein, rat)
RN  - 0 (S100 Proteins)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Action Potentials
MH  - Animals
MH  - Axons/pathology/*physiology
MH  - Brain-Derived Neurotrophic Factor/biosynthesis
MH  - Carrier Proteins/biosynthesis
MH  - Chitosan/chemistry
MH  - Disease Models, Animal
MH  - *Electric Stimulation
MH  - Electrophysiology/methods
MH  - Male
MH  - Myelin P0 Protein/biosynthesis
MH  - Myelin Sheath/*metabolism
MH  - Nerve Regeneration/*physiology
MH  - Nerve Tissue Proteins
MH  - Neurons/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - S100 Proteins/biosynthesis
MH  - Sciatic Nerve/pathology
MH  - Up-Regulation
PMC - PMC3380893
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/06/28 06:00
MHDA- 2012/12/15 06:00
PMCR- 2012/06/21
CRDT- 2012/06/28 06:00
PHST- 2011/10/03 00:00 [received]
PHST- 2012/05/23 00:00 [accepted]
PHST- 2012/06/28 06:00 [entrez]
PHST- 2012/06/28 06:00 [pubmed]
PHST- 2012/12/15 06:00 [medline]
PHST- 2012/06/21 00:00 [pmc-release]
AID - PONE-D-11-19433 [pii]
AID - 10.1371/journal.pone.0039526 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(6):e39526. doi: 10.1371/journal.pone.0039526. Epub 2012 Jun 21.