PMID- 22738368
OWN - NLM
STAT- MEDLINE
DCOM- 20121212
LR  - 20120813
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 80
IP  - 3
DP  - 2012 Sep
TI  - Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal 
      antibodies.
PG  - 231-8
LID - 10.1111/j.1399-0039.2012.01922.x [doi]
AB  - Human leukocyte antigen (HLA)-G is a non-classical HLA class I molecule thought 
      to play a key role in maternal-fetal tolerance. Although initial studies 
      suggested that HLA-G expression is restricted to extravillous cytotrophoblasts, 
      expression was subsequently reported in a wide variety of other human tissues and 
      tumor cells. However, consensus as to the validity of these collective findings 
      has proven difficult because the antibodies used to define the temporal and 
      spatial expression patterns of HLA-G remain incompletely characterized. The aim 
      of our study was to reassess two of the most widely used HLA-G antibodies 
      (MEM-G/9 and 4H84) in HLA-G-positive (JEG-3 and HLA-G transduced) and -negative 
      (dermal fibroblast, mesenchymal stem cell, K562, and Jurkat) lines using flow 
      cytometry, immunofluorescence, and western blotting. We found that MEM-G/9 
      recognized HLA-G3 by flow cytometry, indicating that its epitope is present on 
      the alpha1 domain of HLA-G. Although 4H84 preferably recognized unfolded HLA-G-free 
      chains, it showed strong non-specificity under certain methodological conditions.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Zhao, L
AU  - Zhao L
AD  - Escape Therapeutics, Inc., San Jose, CA 95138, USA.
FAU - Teklemariam, T
AU  - Teklemariam T
FAU - Hantash, B M
AU  - Hantash BM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120628
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Protein Isoforms)
RN  - 0 (beta 2-Microglobulin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity/*immunology
MH  - Cell Line, Tumor
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - HLA-G Antigens/chemistry/*immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Isoforms/immunology
MH  - Transduction, Genetic
MH  - beta 2-Microglobulin/immunology
EDAT- 2012/06/29 06:00
MHDA- 2012/12/13 06:00
CRDT- 2012/06/29 06:00
PHST- 2011/08/04 00:00 [received]
PHST- 2012/04/19 00:00 [revised]
PHST- 2012/06/12 00:00 [accepted]
PHST- 2012/06/29 06:00 [entrez]
PHST- 2012/06/29 06:00 [pubmed]
PHST- 2012/12/13 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01922.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Sep;80(3):231-8. doi: 10.1111/j.1399-0039.2012.01922.x. 
      Epub 2012 Jun 28.