PMID- 22738700
OWN - NLM
STAT- MEDLINE
DCOM- 20121102
LR  - 20190918
IS  - 1130-0108 (Print)
IS  - 1130-0108 (Linking)
VI  - 104
IP  - 6
DP  - 2012 Jun
TI  - Mitogen activated protein kinases blockade improves lipopolysaccharide-induced 
      ileal motor disturbances.
PG  - 305-9
AB  - BACKGROUND: several diseases such as sepsis can affect the ileum. 
      Lipopolysaccharide (LPS), an endotoxin present in the cell wall of gram negative 
      bacteria, is a causative agent of sepsis. OBJECTIVES: the aims of this study 
      were: a) to investigate the role of mitogen activated protein kinases (MAPKs) in 
      the effect of LPS on the acetylcholine-induced contractions of rabbit ileum; and 
      b) to study the localization of MAPKs in the ileum. MATERIAL AND METHODS: ileal 
      contractility was studied in an organ bath and MAPKs were localized by 
      immunohistochemistry. RESULTS: acetylcholine-induced contractions decreased with 
      LPS. SB203580, SP600125 and U0126 blocked the effect of LPS on the 
      acetylcholine-induced contractions. Phosphorylated p38 and ERK were detected in 
      neurons of myenteric plexus and Phosphorylated p38 and JNK in smooth muscle cells 
      of ileum. CONCLUSION: we can suggest that p38, JNK, and ERK MAPKs are involved in 
      the mechanism of action of LPS in the ileum.
FAU - Gonzalo, Sergio
AU  - Gonzalo S
AD  - Department of Pharmacology and Physiology, Unit of Physiology, Facultad de 
      Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.
FAU - Grasa, Laura
AU  - Grasa L
FAU - Hernandez, Ligia Veronica
AU  - Hernandez LV
FAU - Arruebo, Maria Pilar
AU  - Arruebo MP
FAU - Plaza, Miguel Angel
AU  - Plaza MA
FAU - Murillo, Maria Divina
AU  - Murillo MD
LA  - eng
LA  - spa
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - Rev Esp Enferm Dig
JT  - Revista espanola de enfermedades digestivas
JID - 9007566
RN  - 0 (Anthracenes)
RN  - 0 (Biomarkers)
RN  - 0 (Butadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Imidazoles)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nitriles)
RN  - 0 (Pyridines)
RN  - 0 (U 0126)
RN  - 0 (lipopolysaccharide, Escherichia coli O111 B4)
RN  - 1TW30Y2766 (pyrazolanthrone)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - N9YNS0M02X (Acetylcholine)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Acetylcholine/physiology
MH  - Animals
MH  - Anthracenes/pharmacology
MH  - Biomarkers/metabolism
MH  - Butadienes/pharmacology
MH  - Enzyme Inhibitors/*pharmacology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Ileum/drug effects/metabolism/microbiology/*physiopathology
MH  - Imidazoles/pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Lipopolysaccharides/*adverse effects
MH  - Male
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Muscle Contraction/drug effects/*physiology
MH  - Nitriles/pharmacology
MH  - Pyridines/pharmacology
MH  - Rabbits
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2012/06/29 06:00
MHDA- 2012/11/03 06:00
CRDT- 2012/06/29 06:00
PHST- 2012/06/29 06:00 [entrez]
PHST- 2012/06/29 06:00 [pubmed]
PHST- 2012/11/03 06:00 [medline]
AID - 10.4321/s1130-01082012000600004 [doi]
PST - ppublish
SO  - Rev Esp Enferm Dig. 2012 Jun;104(6):305-9. doi: 10.4321/s1130-01082012000600004.