PMID- 22739441
OWN - NLM
STAT- MEDLINE
DCOM- 20121002
LR  - 20181201
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 432
IP  - 1
DP  - 2012 Oct 10
TI  - Chimeric viruses containing the N-terminal ectodomains of GP5 and M proteins of 
      porcine reproductive and respiratory syndrome virus do not change the cellular 
      tropism of equine arteritis virus.
PG  - 99-109
LID - 10.1016/j.virol.2012.05.022 [doi]
AB  - Equine arteritis virus (EAV) and porcine reproductive and respiratory syndrome 
      virus (PRRSV) are members of family Arteriviridae; they are highly species 
      specific and differ significantly in cellular tropism in cultured cells. In this 
      study we examined the role of the two major envelope proteins (GP5 and M) of EAV 
      and PRRSV in determining their cellular tropism. We generated three viable 
      EAV/PRRSV chimeric viruses by swapping the N-terminal ectodomains of these two 
      proteins from PRRSV IA1107 strain into an infectious cDNA clone of EAV (rMLVB4/5 
      GP5ecto, rMLVB4/5/6 Mecto and rMLVB4/5/6 GP5&Mecto). The three chimeric viruses 
      could only infect EAV susceptible cell lines but not PRRSV susceptible cells in 
      culture. Therefore, these data unequivocally demonstrate that the ectodomains of 
      GP5 and M are not the major determinants of cellular tropism, further supporting 
      the recent findings that the minor envelope proteins are the critical proteins in 
      mediating cellular tropism (Tian et al., 2012).
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Lu, Zhengchun
AU  - Lu Z
AD  - Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, 
      University of Kentucky, Lexington, KY 40546, United States.
FAU - Zhang, Jianqiang
AU  - Zhang J
FAU - Huang, Chengjin M
AU  - Huang CM
FAU - Go, Young Yun
AU  - Go YY
FAU - Faaberg, Kay S
AU  - Faaberg KS
FAU - Rowland, Raymond R R
AU  - Rowland RR
FAU - Timoney, Peter J
AU  - Timoney PJ
FAU - Balasuriya, Udeni B R
AU  - Balasuriya UB
LA  - eng
SI  - GENBANK/JQ844153
SI  - GENBANK/JQ844154
SI  - GENBANK/JQ844155
SI  - GENBANK/JQ844156
SI  - GENBANK/JQ844157
SI  - GENBANK/JQ844158
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120626
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Complementary)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA, Complementary
MH  - Equartevirus/genetics/*physiology
MH  - Horses
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/genetics
MH  - Protein Structure, Tertiary
MH  - Recombination, Genetic
MH  - Sequence Analysis, DNA
MH  - Swine
MH  - Viral Envelope Proteins/*genetics/metabolism
MH  - Viral Matrix Proteins/*genetics/metabolism
MH  - *Viral Tropism
EDAT- 2012/06/29 06:00
MHDA- 2012/10/04 06:00
CRDT- 2012/06/29 06:00
PHST- 2012/04/17 00:00 [received]
PHST- 2012/05/15 00:00 [revised]
PHST- 2012/05/27 00:00 [accepted]
PHST- 2012/06/29 06:00 [entrez]
PHST- 2012/06/29 06:00 [pubmed]
PHST- 2012/10/04 06:00 [medline]
AID - S0042-6822(12)00273-5 [pii]
AID - 10.1016/j.virol.2012.05.022 [doi]
PST - ppublish
SO  - Virology. 2012 Oct 10;432(1):99-109. doi: 10.1016/j.virol.2012.05.022. Epub 2012 
      Jun 26.