PMID- 22740324
OWN - NLM
STAT- MEDLINE
DCOM- 20130409
LR  - 20181202
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 6
IP  - 10
DP  - 2012 Nov
TI  - Whatever their differentiation status, human progenitor derived - or mature - 
      endothelial cells induce osteoblastic differentiation of bone marrow stromal 
      cells.
PG  - e51-60
LID - 10.1002/term.1539 [doi]
AB  - Association of the bone-forming osteoblasts (OBs) and vascular endothelial cells 
      (ECs) into a biomaterial composite provides a live bone graft substitute that can 
      repair the bone defect when implanted. An intimate functional relationship exists 
      between these cell types. This communication is crucial to the coordinated cell 
      behaviour necessary for bone development and remodelling. Previous studies have 
      shown that direct co-culture of primary human osteoprogenitors (HOPs) with 
      primary human umbilical vein endothelial cells (HUVECs) stimulates HOPs 
      differentiation and induces tubular-like networks. The present work aims to test 
      the use of human bone marrow stromal cells (HBMSCs) co-cultured with human 
      endothelial progenitor cells in order to assess whether progenitor-derived ECs 
      (PDECs) could support osteoblastic differentiation as mature ECs do. Indeed, data 
      generated from the literature by different laboratories considering these 
      co-culture systems appear difficult to compare. Monocultures of HUVECs, HOPs, 
      HBMSCs (in a non-orientated lineage), PDECs (from cord blood) were used as 
      controls and four combinations of co-cultures were undertaken: HBMSCs-PDECs, 
      HBMSCs-HUVECs, HOPs-PDECs, HOPs-HUVECs with ECs (mature or progenitor) for 6 h to 
      7 days. At the end of the chosen co-culture time, intracellular alkaline 
      phosphatase (ALP) activity was detected in HOPs and HBMSCs and quantified in cell 
      extracts. Quantitative real-time polymerase chain reaction (qPCR) of ALP was 
      performed over time and vascular endothelial growth factor (VEGF) was measured. 
      After 21 days, calcium deposition was observed, comparing mono- and co-cultures. 
      We confirm that ECs induce osteoblastic differentiation of mesenchymal stem cells 
      in vitro. Moreover, HUVECs can be replaced by PDECs, the latter being of great 
      interest in tissue engineering.
CI  - Copyright (c) 2012 John Wiley & Sons, Ltd.
FAU - Thebaud, N B
AU  - Thebaud NB
AD  - INSERM, U1026, F-33000, Bordeaux, France.
FAU - Siadous, R
AU  - Siadous R
FAU - Bareille, R
AU  - Bareille R
FAU - Remy, M
AU  - Remy M
FAU - Daculsi, R
AU  - Daculsi R
FAU - Amedee, J
AU  - Amedee J
FAU - Bordenave, L
AU  - Bordenave L
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
DEP - 20120628
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Alkaline Phosphatase/biosynthesis
MH  - *Calcification, Physiologic
MH  - Calcium/metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells/cytology/*metabolism
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Middle Aged
MH  - Osteoblasts/cytology/*metabolism
MH  - Tissue Engineering/methods
EDAT- 2012/06/29 06:00
MHDA- 2013/04/10 06:00
CRDT- 2012/06/29 06:00
PHST- 2011/10/27 00:00 [received]
PHST- 2012/02/16 00:00 [revised]
PHST- 2012/04/18 00:00 [accepted]
PHST- 2012/06/29 06:00 [entrez]
PHST- 2012/06/29 06:00 [pubmed]
PHST- 2013/04/10 06:00 [medline]
AID - 10.1002/term.1539 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2012 Nov;6(10):e51-60. doi: 10.1002/term.1539. Epub 2012 
      Jun 28.