PMID- 22740396
OWN - NLM
STAT- MEDLINE
DCOM- 20121121
LR  - 20211021
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 86
IP  - 18
DP  - 2012 Sep
TI  - Restricted varicella-zoster virus transcription in human trigeminal ganglia 
      obtained soon after death.
PG  - 10203-6
LID - 10.1128/JVI.01331-12 [doi]
AB  - We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently 
      infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging 
      from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV 
      transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P 
      = 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer 
      PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 
      transcript levels in human cadaver TG increased with longer PMI.
FAU - Ouwendijk, Werner J D
AU  - Ouwendijk WJ
AD  - Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
FAU - Choe, Alexander
AU  - Choe A
FAU - Nagel, Maria A
AU  - Nagel MA
FAU - Gilden, Don
AU  - Gilden D
FAU - Osterhaus, Albert D M E
AU  - Osterhaus AD
FAU - Cohrs, Randall J
AU  - Cohrs RJ
FAU - Verjans, Georges M G M
AU  - Verjans GM
LA  - eng
GR  - K08 NS067070/NS/NINDS NIH HHS/United States
GR  - P01 AG032958/AG/NIA NIH HHS/United States
GR  - AG032958/AG/NIA NIH HHS/United States
GR  - NS067070/NS/NINDS NIH HHS/United States
GR  - R37 AG006127/AG/NIA NIH HHS/United States
GR  - R01 AG006127/AG/NIA NIH HHS/United States
GR  - AG006127/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120627
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (immediate early protein 63, Human herpesvirus 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Herpesvirus 3, Human/*genetics/isolation & purification
MH  - Humans
MH  - Immediate-Early Proteins/genetics
MH  - Male
MH  - Middle Aged
MH  - Postmortem Changes
MH  - RNA, Viral/genetics/isolation & purification
MH  - Real-Time Polymerase Chain Reaction
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Transcriptome
MH  - Trigeminal Ganglion/*virology
MH  - Viral Envelope Proteins/genetics
PMC - PMC3446590
EDAT- 2012/06/29 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/03/01
CRDT- 2012/06/29 06:00
PHST- 2012/06/29 06:00 [entrez]
PHST- 2012/06/29 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - JVI.01331-12 [pii]
AID - 01331-12 [pii]
AID - 10.1128/JVI.01331-12 [doi]
PST - ppublish
SO  - J Virol. 2012 Sep;86(18):10203-6. doi: 10.1128/JVI.01331-12. Epub 2012 Jun 27.