PMID- 22744622
OWN - NLM
STAT- MEDLINE
DCOM- 20121108
LR  - 20181201
IS  - 2299-8306 (Electronic)
IS  - 0423-104X (Linking)
VI  - 63
IP  - 3
DP  - 2012
TI  - Oxytocin and vasopressin secretion from the rat hypothalamo-neurohypophysial 
      system is stimulated by triptorelin.
PG  - 176-81
AB  - INTRODUCTION: Several observations have suggested that the secretion of 
      neurohypophysial hormones could be modified by gonadotropin- releasing hormone 
      (GnRH). Since, in medical practice, more often than GnRH itself, its analogues 
      are used, the present study was undertaken to investigate the influence of the 
      GnRH agonist - triptorelin on oxytocin (OT) and vasopressin (AVP) release from 
      the rat hypothalamo-neurohypophysial (H-N) system both in vitro and in vivo. 
      MATERIALS AND METHODS: Male rats served as donors of the H-N explants, which were 
      placed in 1 mL of Krebs-Ringer fluid (nKRF) and incubated successively in: 1 - 
      nKRF (B1); 2 - incubation fluid as B1 enriched with an excess amount (56 mM) of 
      K(+) (S1); 3 - incubation fluid as B1 enriched with an appropriate concentration 
      of triptorelin, i.e., 10(-11) - 10(-5) M (B2); and 4 - incubation fluid as S1 
      enriched with the same concentrations of triptorelin (S2). After 20 minutes of 
      incubation, each medium (B1, S1, B2, S2) was collected and frozen before OT and 
      AVP estimation by the RIA. During in vivo experiment, animals were infused 
      intracerebroventricularly (icv) with triptorelin, at a concentration of 10(-7) M, 
      and 20 minutes later they were decapitated. The neurohypophysis was dissected 
      from the brain and blood plasma samples were collected and frozen for further OT 
      and AVP RIA assays. RESULTS: The GnRH agonist - triptorelin stimulates both OT 
      and AVP release from isolated H-N system at concentrations of 10(-9)-10(-5) M. 
      The strongest effect was displayed by triptorelin at a concentration of 10(-7) M. 
      Under the conditions of K(+) stimulation, triptorelin affects neither OT, nor AVP 
      secretion in vitro. When infused icv, triptorelin, at a concentration of 10(-7) 
      M, significantly stimulated both OT and AVP secretion into the blood. 
      CONCLUSIONS: Triptorelin may play a role as a neuromodulator contributing to the 
      functional regulation of OT and AVP secretion in the rat.
FAU - Juszczak, Marlena
AU  - Juszczak M
AD  - Department of Pathophysiology and Experimental Neuroendocrinology, Chair of 
      Department of General and Experimental Pathology, Medical University of Lodz, 
      Poland. marlena.juszczak@umed.lodz.pl
FAU - Roszczyk, Magdalena
AU  - Roszczyk M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Endokrynol Pol
JT  - Endokrynologia Polska
JID - 0370674
RN  - 08AN7WA2G0 (Triptorelin Pamoate)
RN  - 11000-17-2 (Vasopressins)
RN  - 50-56-6 (Oxytocin)
SB  - IM
MH  - Animals
MH  - Hypothalamo-Hypophyseal System/*metabolism
MH  - Male
MH  - Oxytocin/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Triptorelin Pamoate/*metabolism
MH  - Vasopressins/*metabolism
EDAT- 2012/06/30 06:00
MHDA- 2012/11/09 06:00
CRDT- 2012/06/30 06:00
PHST- 2012/06/30 06:00 [entrez]
PHST- 2012/06/30 06:00 [pubmed]
PHST- 2012/11/09 06:00 [medline]
PST - ppublish
SO  - Endokrynol Pol. 2012;63(3):176-81.