PMID- 22744745
OWN - NLM
STAT- MEDLINE
DCOM- 20131230
LR  - 20230614
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Print)
IS  - 1073-6085 (Linking)
VI  - 54
IP  - 2
DP  - 2013 Jun
TI  - Prokaryotic expression and in vitro functional analysis of IL-1beta and MCP-1 from 
      guinea pig.
PG  - 312-9
LID - 10.1007/s12033-012-9574-z [doi]
AB  - The Guinea pig (Cavia porcellus) is an excellent animal model for studying human 
      tuberculosis (TB) and also for a number of other infectious and non-infectious 
      diseases. One of the major roadblocks in effective utilization of this animal 
      model is the lack of readily available immunological reagents. In order to 
      address this issue, guinea pig interleukin 1 beta (IL-1beta) and monocyte 
      chemoattractant protein-1 (MCP-1) were efficiently cloned and expressed in a 
      prokaryotic expression vector, and the expressed proteins in soluble form from 
      both the genes were confirmed by N-terminal sequencing. The biological activity 
      of recombinant guinea pig IL-1beta was demonstrated by its ability to drive 
      proliferation in thymocytes, and the recombinant guinea pig MCP-1 exhibited 
      chemotactic activity for guinea pig resident peritoneal macrophages. These 
      biologically active recombinant guinea pig proteins will facilitate an in-depth 
      understanding of the role they play in the immune responses of the guinea pig to 
      TB and other diseases.
FAU - Dirisala, Vijaya R
AU  - Dirisala VR
AD  - Department of Microbial and Molecular Pathogenesis, Texas A&M Health Science 
      Center, College Station, TX, USA. dirisala@medicine.tamhsc.edu
FAU - Jeevan, Amminikutty
AU  - Jeevan A
FAU - Ly, Lan H
AU  - Ly LH
FAU - McMurray, David N
AU  - McMurray DN
LA  - eng
GR  - HHSN266200400091C/AI/NIAID NIH HHS/United States
GR  - N01AI40091/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Chemokine CCL2/biosynthesis/*genetics/metabolism
MH  - Cloning, Molecular/methods
MH  - Genetic Vectors/genetics
MH  - Guinea Pigs
MH  - Interleukin-1beta/biosynthesis/*genetics/metabolism
MH  - Macrophages, Peritoneal/metabolism
MH  - Recombinant Proteins/biosynthesis/genetics/metabolism
MH  - Thymocytes/metabolism
PMC - PMC3594633
MID - NIHMS405871
EDAT- 2012/06/30 06:00
MHDA- 2014/01/01 06:00
PMCR- 2014/06/01
CRDT- 2012/06/30 06:00
PHST- 2012/06/30 06:00 [entrez]
PHST- 2012/06/30 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2014/06/01 00:00 [pmc-release]
AID - 10.1007/s12033-012-9574-z [doi]
PST - ppublish
SO  - Mol Biotechnol. 2013 Jun;54(2):312-9. doi: 10.1007/s12033-012-9574-z.