PMID- 22745577
OWN - NLM
STAT- MEDLINE
DCOM- 20121025
LR  - 20211021
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 8
IP  - 6
DP  - 2012
TI  - Genome-wide association study of porcine hematological parameters in a Large 
      White x Minzhu F2 resource population.
PG  - 870-81
LID - 10.7150/ijbs.4027 [doi]
AB  - Hematological traits, which are important indicators of immune function in 
      animals, have been commonly examined as biomarkers of disease and disease 
      severity in humans and animals. Genome-wide significant quantitative trait loci 
      (QTLs) provide important information for use in breeding programs of animals such 
      as pigs. QTLs for hematological parameters (hematological traits) have been 
      detected in pig chromosomes, although these are often mapped by linkage analysis 
      to large intervals making identification of the underlying mutation problematic. 
      Single nucleotide polymorphisms (SNPs) are the common form of genetic variation 
      among individuals and are thought to account for the majority of inherited 
      traits. In this study, a genome-wide association study (GWAS) was performed to 
      detect regions of association with hematological traits in a three-generation 
      resource population produced by intercrossing Large White boars and Minzhu sows 
      during the period from 2007 to 2011. Illumina PorcineSNP60 BeadChip technology 
      was used to genotype each animal and seven hematological parameters were measured 
      (hematocrit (HCT), hemoglobin (HGB), mean corpuscular hemoglobin (MCH), mean 
      corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), red 
      blood cell count (RBC) and red blood cell volume distribution width (RDW)). Data 
      were analyzed in a three step Genome-wide Rapid Association using the Mixed Model 
      and Regression-Genomic Control (GRAMMAR-GC) method. A total of 62 genome-wide 
      significant and three chromosome-wide significant SNPs associated with 
      hematological parameters were detected in this GWAS. Seven and five SNPs were 
      associated with HCT and HGB, respectively. These SNPs were all located within the 
      region of 34.6-36.5 Mb on SSC7. Four SNPs within the region of 43.7-47.0 Mb and 
      fifty-five SNPs within the region of 42.2-73.8 Mb on SSC8 showed significant 
      association with MCH and MCV, respectively. At chromosome-wide significant level, 
      one SNP at 29.2 Mb on SSC1 and two SNPs within the region of 26.0-26.2 Mb were 
      found to be significantly associated with RBC and RDW, respectively. Many of the 
      SNPs were located within previously reported QTL regions and appeared to narrow 
      down the regions compared with previously described QTL intervals. In current 
      research, a total of seven significant SNPs were found within six candidate genes 
      SCUBE3, KDR, TDO, IGFBP7, ADAMTS3 and AFP. In addition, the KIT gene, which has 
      been previously reported to relate to hematological parameters, was located 
      within the region significantly associated with MCH and MCV and could be a 
      candidate gene. These results of this study may lead to a better understanding of 
      the molecular mechanisms of hematological parameters in pigs.
FAU - Luo, Weizhen
AU  - Luo W
AD  - Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of 
      Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Chen, Shaokang
AU  - Chen S
FAU - Cheng, Duxue
AU  - Cheng D
FAU - Wang, Ligang
AU  - Wang L
FAU - Li, Yong
AU  - Li Y
FAU - Ma, Xiaojun
AU  - Ma X
FAU - Song, Xin
AU  - Song X
FAU - Liu, Xin
AU  - Liu X
FAU - Li, Wen
AU  - Li W
FAU - Liang, Jing
AU  - Liang J
FAU - Yan, Hua
AU  - Yan H
FAU - Zhao, Kebin
AU  - Zhao K
FAU - Wang, Chuduan
AU  - Wang C
FAU - Wang, Lixian
AU  - Wang L
FAU - Zhang, Longchao
AU  - Zhang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120615
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Hemoglobins)
RN  - 0 (Insulin-Like Growth Factor Binding Proteins)
RN  - EC 3.4.24.14 (Procollagen N-Endopeptidase)
SB  - IM
MH  - Animals
MH  - Calcium-Binding Proteins/genetics
MH  - Genome-Wide Association Study/*methods
MH  - Hemoglobins/genetics
MH  - Insulin-Like Growth Factor Binding Proteins/genetics
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Procollagen N-Endopeptidase/genetics
MH  - Quantitative Trait Loci/genetics
MH  - Swine
PMC - PMC3385009
OTO - NOTNLM
OT  - genome-wide association study
OT  - hematological parameters
OT  - porcine
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2012/06/30 06:00
MHDA- 2012/10/26 06:00
PMCR- 2012/01/01
CRDT- 2012/06/30 06:00
PHST- 2011/12/30 00:00 [received]
PHST- 2012/05/15 00:00 [accepted]
PHST- 2012/06/30 06:00 [entrez]
PHST- 2012/06/30 06:00 [pubmed]
PHST- 2012/10/26 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - ijbsv08p0870 [pii]
AID - 10.7150/ijbs.4027 [doi]
PST - ppublish
SO  - Int J Biol Sci. 2012;8(6):870-81. doi: 10.7150/ijbs.4027. Epub 2012 Jun 15.