PMID- 22745651
OWN - NLM
STAT- MEDLINE
DCOM- 20121029
LR  - 20240315
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 1
DP  - 2012
TI  - A piezoelectric immunosensor using hybrid self-assembled monolayers for detection 
      of Schistosoma japonicum.
PG  - e30779
LID - 10.1371/journal.pone.0030779 [doi]
LID - e30779
AB  - BACKGROUND: The parasite Schistosoma japonicum causes schistosomiasis disease, 
      which threatens human life and hampers economic and social development in some 
      Asian countries. An important lesson learned from efforts to reduce the 
      occurrence of schistosomiasis is that the diagnostic approach must be altered as 
      further progress is made towards the control and ultimate elimination of the 
      disease. METHODOLOGY/PRINCIPAL FINDINGS: Using mixed self-assembled monolayer 
      membrane (mixed SAM) technology, a mixture of mercaptopropionic acid (MPA) and 
      mercaptoethanol (ME) was self-assembled on the surface of quartz crystals by 
      gold-sulphur-bonds. Soluble egg antigens (SEA) of S. japonicum were then 
      cross-linked to the quartz crystal using a special coupling agent. As compared 
      with the traditional single self-assembled monolayer immobilization method, S. 
      japonicum antigen (SjAg) immobilization using mixed self-assembled monolayers 
      exhibits much greater immunoreactivity. Under optimal experimental conditions, 
      the detection range is 1:1500 to 1:60 (infected rabbit serum dilution ratios). We 
      measured several infected rabbit serum samples with varying S. japonicum antibody 
      (SjAb) concentrations using both immunosensor and ELISA techniques and then 
      produced a correlation analysis. The correlation coefficients reached 0.973. 
      CONCLUSIONS/SIGNIFICANCE: We have developed a new, simple, sensitive, and 
      reusable piezoelectric immunosensor that directly detects SjAb in the serum. This 
      method may represent an alternative to the current diagnostic methods for S. 
      japonicum infection in the clinical laboratory or for analysis outside the 
      laboratory.
FAU - Wang, Shiping
AU  - Wang S
AD  - Key Laboratory of Schistosomiasis in Hunan, Department of Parasitology, Xiangya 
      Medical College, Central South University, Changsha, China. spwang@126.com
FAU - Yin, Tieqiu
AU  - Yin T
FAU - Zeng, Shaohua
AU  - Zeng S
FAU - Che, Hongli
AU  - Che H
FAU - Yang, Feifei
AU  - Yang F
FAU - Chen, Xiuchun
AU  - Chen X
FAU - Shen, Guoli
AU  - Shen G
FAU - Wu, Zhaoyang
AU  - Wu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120127
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (Membranes, Artificial)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/*blood
MH  - Antigens, Helminth/immunology
MH  - Biosensing Techniques/*methods
MH  - Humans
MH  - Membranes, Artificial
MH  - Rabbits
MH  - Schistosoma japonicum/*immunology
MH  - Schistosomiasis/*diagnosis
MH  - Sensitivity and Specificity
PMC - PMC3383785
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/06/30 06:00
MHDA- 2012/10/30 06:00
PMCR- 2012/01/27
CRDT- 2012/06/30 06:00
PHST- 2011/06/01 00:00 [received]
PHST- 2011/12/20 00:00 [accepted]
PHST- 2012/06/30 06:00 [entrez]
PHST- 2012/06/30 06:00 [pubmed]
PHST- 2012/10/30 06:00 [medline]
PHST- 2012/01/27 00:00 [pmc-release]
AID - PONE-D-11-09285 [pii]
AID - 10.1371/journal.pone.0030779 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(1):e30779. doi: 10.1371/journal.pone.0030779. Epub 2012 Jan 27.