PMID- 22745844
OWN - NLM
STAT- MEDLINE
DCOM- 20120928
LR  - 20220309
IS  - 1935-2735 (Electronic)
IS  - 1935-2727 (Print)
IS  - 1935-2727 (Linking)
VI  - 6
IP  - 6
DP  - 2012
TI  - Neutrophil paralysis in Plasmodium vivax malaria.
PG  - e1710
LID - 10.1371/journal.pntd.0001710 [doi]
LID - e1710
AB  - BACKGROUND: The activation of innate immune responses by Plasmodium vivax results 
      in activation of effector cells and an excessive production of pro-inflammatory 
      cytokines that may culminate in deleterious effects. Here, we examined the 
      activation and function of neutrophils during acute episodes of malaria. 
      MATERIALS AND METHODS: Blood samples were collected from P. vivax-infected 
      patients at admission (day 0) and 30-45 days after treatment with chloroquine and 
      primaquine. Expression of activation markers and cytokine levels produced by 
      highly purified monocytes and neutrophils were measured by the Cytometric Bead 
      Assay. Phagocytic activity, superoxide production, chemotaxis and the presence of 
      G protein-coupled receptor (GRK2) were also evaluated in neutrophils from malaria 
      patients. PRINCIPAL FINDINGS: Both monocytes and neutrophils from P. 
      vivax-infected patients were highly activated. While monocytes were found to be 
      the main source of cytokines in response to TLR ligands, neutrophils showed 
      enhanced phagocytic activity and superoxide production. Interestingly, 
      neutrophils from the malaria patients expressed high levels of GRK2, low levels 
      of CXCR2, and displayed impaired chemotaxis towards IL-8 (CXCL8). CONCLUSION: 
      Activated neutrophils from malaria patients are a poor source of pro-inflammatory 
      cytokines and display reduced chemotactic activity, suggesting a possible 
      mechanism for an enhanced susceptibility to secondary bacterial infection during 
      malaria.
FAU - Leoratti, Fabiana Maria de Souza
AU  - Leoratti FM
AD  - Laboratorio de Imunopatologia, Centro de Pesquisas Rene Rachou, Fundacao Oswaldo 
      Cruz, Belo Horizonte, Minas Gerais, Brazil.
FAU - Trevelin, Silvia Cellone
AU  - Trevelin SC
FAU - Cunha, Fernando Queiroz
AU  - Cunha FQ
FAU - Rocha, Bruno Coelho
AU  - Rocha BC
FAU - Costa, Pedro Augusto Carvalho
AU  - Costa PA
FAU - Gravina, Humberto Doriguetto
AU  - Gravina HD
FAU - Tada, Mauro Shugiro
AU  - Tada MS
FAU - Pereira, Dhelio Batista
AU  - Pereira DB
FAU - Golenbock, Douglas Taylor
AU  - Golenbock DT
FAU - Antonelli, Lis Ribeiro do Valle
AU  - Antonelli LR
FAU - Gazzinelli, Ricardo T
AU  - Gazzinelli RT
LA  - eng
GR  - R01 AI079293/AI/NIAID NIH HHS/United States
GR  - R21 AI080907/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120626
PL  - United States
TA  - PLoS Negl Trop Dis
JT  - PLoS neglected tropical diseases
JID - 101291488
RN  - 0 (Cytokines)
RN  - 11062-77-4 (Superoxides)
RN  - EC 2.7.11.15 (GRK2 protein, human)
RN  - EC 2.7.11.16 (G-Protein-Coupled Receptor Kinase 2)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Chemotaxis
MH  - Cytokines/*biosynthesis
MH  - Female
MH  - Flow Cytometry
MH  - G-Protein-Coupled Receptor Kinase 2/biosynthesis
MH  - Humans
MH  - Malaria, Vivax/complications/*immunology/*pathology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/immunology/parasitology
MH  - Neutrophils/*immunology/*parasitology
MH  - Phagocytosis
MH  - Plasmodium vivax/*immunology/*pathogenicity
MH  - Superoxides/metabolism
MH  - Young Adult
PMC - PMC3383745
COIS- The authors have declared that no competing interests exist.
EDAT- 2012/06/30 06:00
MHDA- 2012/09/29 06:00
PMCR- 2012/06/26
CRDT- 2012/06/30 06:00
PHST- 2012/02/17 00:00 [received]
PHST- 2012/05/13 00:00 [accepted]
PHST- 2012/06/30 06:00 [entrez]
PHST- 2012/06/30 06:00 [pubmed]
PHST- 2012/09/29 06:00 [medline]
PHST- 2012/06/26 00:00 [pmc-release]
AID - PNTD-D-12-00278 [pii]
AID - 10.1371/journal.pntd.0001710 [doi]
PST - ppublish
SO  - PLoS Negl Trop Dis. 2012;6(6):e1710. doi: 10.1371/journal.pntd.0001710. Epub 2012 
      Jun 26.