PMID- 22747755
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20211021
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Print)
IS  - 0275-6382 (Linking)
VI  - 41
IP  - 3
DP  - 2012 Sep
TI  - Acute myeloblastic leukemia with associated BCR-ABL translocation in a dog.
PG  - 362-368
LID - 10.1111/j.1939-165X.2012.00450.x [doi]
AB  - An 8-year-old male neutered Labrador Retriever was referred to the University of 
      Wisconsin Veterinary Medical Teaching Hospital with a presumptive diagnosis of 
      leukemia. Hematologic abnormalities included normal neutrophil count with a left 
      shift, monocytosis, eosinophilia, thrombocytopenia, and circulating immature 
      mononuclear cells. Bone marrow was effaced by immature hematopoietic cells of 
      various morphologic appearances. In addition, large multinucleated cells were 
      observed frequently. Flow cytometric analysis of nucleated cells in blood 
      revealed 34% CD34(+) cells, consistent with acute leukemia. By immunocytochemical 
      analysis of cells in blood and bone marrow, some mononuclear cells expressed 
      CD18, myeloperoxidase, and CD11b, indicating myeloid origin; some, but not all, 
      large multinucleated cells expressed CD117 and CD42b, the latter supporting 
      megakaryocytic lineage. The diagnosis was acute myeloblastic leukemia without 
      maturation (AML-M1). To identify genetic aberrations associated with this 
      malignancy, cells from formalin-fixed paraffin-embedded bone marrow were analyzed 
      cytogenetically by multicolor fluorescence in situ hybridization (FISH). 
      Co-localization of bacterial artificial chromosome (BAC) containing BCR and ABL 
      was evident in 32% of cells. This confirmed the presence of the canine BCR-ABL 
      translocation or Raleigh chromosome. In people, the analogous translocation or 
      Philadelphia chromosome is characteristic of chronic myelogenous leukemia (CML) 
      and is rarely reported in AML. BCR-ABL translocation also has been identified in 
      dogs with CML; however, to our knowledge this is the first report of AML with a 
      BCR-ABL translocation in a domestic animal.
CI  - (c) 2012 American Society for Veterinary Clinical Pathology.
FAU - Figueiredo, Josely F
AU  - Figueiredo JF
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, University 
      of Wisconsin, Madison, WI, USA.
FAU - Culver, Sarah
AU  - Culver S
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, Raleigh, NC, USA.
FAU - Behling-Kelly, Erica
AU  - Behling-Kelly E
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, University 
      of Wisconsin, Madison, WI, USA.
FAU - Breen, Matthew
AU  - Breen M
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, Raleigh, NC, USA.
AD  - Center for Comparative Medicine and Translational Research, North Carolina State 
      University, Raleigh, NC, USA.
AD  - Lineberger Comprehensive Cancer Center, University of North Carolina, Raleigh, 
      NC, USA.
FAU - Friedrichs, Kristen R
AU  - Friedrichs KR
AD  - Department of Pathobiological Sciences, School of Veterinary Medicine, University 
      of Wisconsin, Madison, WI, USA.
LA  - eng
GR  - T35 OD011070/OD/NIH HHS/United States
GR  - T35 RR025837/RR/NCRR NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
DEP - 20120629
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Animals
MH  - Bone Marrow/pathology
MH  - Cytogenetic Analysis/veterinary
MH  - Dog Diseases/*genetics/immunology/pathology
MH  - Dogs
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Hematologic Tests/veterinary
MH  - Immunophenotyping/veterinary
MH  - In Situ Hybridization, Fluorescence/veterinary
MH  - Leukemia, Myeloid, Acute/genetics/immunology/pathology/*veterinary
MH  - Male
MH  - Translocation, Genetic/*genetics
PMC - PMC4501036
MID - NIHMS702425
EDAT- 2012/07/04 06:00
MHDA- 2014/08/27 06:00
PMCR- 2015/07/14
CRDT- 2012/07/04 06:00
PHST- 2012/07/04 06:00 [entrez]
PHST- 2012/07/04 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
PHST- 2015/07/14 00:00 [pmc-release]
AID - 10.1111/j.1939-165X.2012.00450.x [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2012 Sep;41(3):362-368. doi: 10.1111/j.1939-165X.2012.00450.x. 
      Epub 2012 Jun 29.