PMID- 22750585
OWN - NLM
STAT- MEDLINE
DCOM- 20130114
LR  - 20120803
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1469
DP  - 2012 Aug 21
TI  - Stem cell-based delivery of brain-derived neurotrophic factor gene in the rat 
      retina.
PG  - 10-23
LID - 10.1016/j.brainres.2012.06.006 [doi]
AB  - As an alternative to a viral vector, the application of stem cells to transfer 
      specific genes is under investigation in various organs. Using this strategy may 
      provide more effective method to supply neurotrophic factor to the 
      neurodegenerative diseases caused by neurotrophic factor deprivation. This study 
      investigated the possibility and efficacy of stem cell-based delivery of the 
      brain-derived neurotrophic factor (BDNF) gene to rat retina. Rat BDNF cDNA was 
      transduced into rat bone marrow mesenchymal stem cells (rMSCs) using a retroviral 
      vector. Its incorporation into the experimental rat retina and the expression of 
      BDNF after intravitreal injection or subretinal injection were detected by 
      real-time PCR, western blot analysis, and immunohistochemical staining. For the 
      incorporated rMSCs, retinal-specific marker staining was performed to investigate 
      the changes in morphology and the characteristics of the stem cells. Transduction 
      of the rMSCs by retrovirus was effective, and the transduced rMSCs expressed high 
      levels of the BDNF gene and protein. The subretinal injection of rMSCs produced 
      rMSC migration and incorporation into the rat retina (about 15.7% incorporation 
      rate), and retinal BDNF mRNA and protein expression was increased at 4 weeks 
      after transplantation. When subretinal injection of rMSCs was applied to 
      axotomized rat retina, it significantly increased the expression of BDNF until 4 
      weeks after transplantation. Some of the transplanted rMSCs exhibited 
      morphological changes, but the retinal-specific marker stain was not sufficient 
      to indicate whether neuronal differentiation had occurred. Using mesenchymal stem 
      cells to deliver the BDNF gene to the retina may provide new treatment for 
      glaucoma.
CI  - Crown Copyright (c) 2012. Published by Elsevier B.V. All rights reserved.
FAU - Park, Hae-Young Lopilly
AU  - Park HY
AD  - Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, 
      College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-ku, 
      Seoul 137-701, Republic of Korea.
FAU - Kim, Jie Hyun
AU  - Kim JH
FAU - Sun Kim, Hwa
AU  - Sun Kim H
FAU - Park, Chan Kee
AU  - Park CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120630
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage/genetics/metabolism
MH  - Cell Differentiation/*drug effects/physiology
MH  - Drug Delivery Systems/*methods
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/*drug effects
EDAT- 2012/07/04 06:00
MHDA- 2013/01/15 06:00
CRDT- 2012/07/04 06:00
PHST- 2011/09/21 00:00 [received]
PHST- 2012/05/18 00:00 [revised]
PHST- 2012/06/06 00:00 [accepted]
PHST- 2012/07/04 06:00 [entrez]
PHST- 2012/07/04 06:00 [pubmed]
PHST- 2013/01/15 06:00 [medline]
AID - S0006-8993(12)01058-X [pii]
AID - 10.1016/j.brainres.2012.06.006 [doi]
PST - ppublish
SO  - Brain Res. 2012 Aug 21;1469:10-23. doi: 10.1016/j.brainres.2012.06.006. Epub 2012 
      Jun 30.