PMID- 22751686
OWN - NLM
STAT- MEDLINE
DCOM- 20121106
LR  - 20211203
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 6
IP  - 3
DP  - 2012 Sep
TI  - Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to 
      the growth and survival of oral cancer cells.
PG  - 675-81
LID - 10.3892/mmr.2012.971 [doi]
AB  - MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene 
      expression by either translational inhibition or mRNA degradation. miRNAs play 
      pivotal roles in physiological functioning and pathological progression. The 
      function of microRNA-99a (miR-99a) in oral squamous cell carcinoma (OSCC) remains 
      unclear. In the present study, we investigated the roles of miR-99a in OSCC 
      development and the underlying mechanisms in 25 cases of primary OSCC tissues and 
      Tca-8113 cells. The cells were analyzed using FACS analysis and western blotting. 
      Results showed that the expression levels of miR-99a were markedly decreased in 
      OSCC tissues compared with the adjacent non-tumor tissues (n=25). The results of 
      in vitro experiments showed that miR-99a mimics significantly inhibited the 
      proliferation of Tca-8113 cells, a tongue squamous carcinoma cell line, and that 
      miR-99a mimics markedly induced the apoptosis of Tca-8113 cells. Furthermore, we 
      demonstrated that mammalian target of rapamycin (mTOR) was directly targeted by 
      miR-99a, as the overexpression of miR-99a in Tca-8113 cells downregulated the 
      protein expression level of mTOR. Thus, our findings suggest that the 
      downregulation of miR-99a in OSCC tissues is associated with tumor development. 
      miR-99a regulates the growth and survival of OSCC cells and may be exploited as a 
      biomarker and therapeutic target for patients with OSCC.
FAU - Yan, Bin
AU  - Yan B
AD  - Department of Oral and Maxillofacial Surgery, Ningbo First Hospital, Ningbo 
      315000, PR China.
FAU - Fu, Qihong
AU  - Fu Q
FAU - Lai, Lihua
AU  - Lai L
FAU - Tao, Xinran
AU  - Tao X
FAU - Fei, Yanghonghong
AU  - Fei Y
FAU - Shen, Jiangen
AU  - Shen J
FAU - Chen, Zuodong
AU  - Chen Z
FAU - Wang, Qingqing
AU  - Wang Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120627
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN99 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Apoptosis/drug effects
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Down-Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - MicroRNAs/*metabolism/pharmacology
MH  - Mouth Neoplasms/*metabolism/pathology
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
EDAT- 2012/07/04 06:00
MHDA- 2012/11/07 06:00
CRDT- 2012/07/04 06:00
PHST- 2012/02/21 00:00 [received]
PHST- 2012/06/20 00:00 [accepted]
PHST- 2012/07/04 06:00 [entrez]
PHST- 2012/07/04 06:00 [pubmed]
PHST- 2012/11/07 06:00 [medline]
AID - 10.3892/mmr.2012.971 [doi]
PST - ppublish
SO  - Mol Med Rep. 2012 Sep;6(3):675-81. doi: 10.3892/mmr.2012.971. Epub 2012 Jun 27.