PMID- 22752913
OWN - NLM
STAT- MEDLINE
DCOM- 20121029
LR  - 20211021
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Linking)
VI  - 50
IP  - 3
DP  - 2012 Jun
TI  - Nucleotide-binding oligomerization domain 2 (Nod2) is dispensable for the innate 
      immune responses of macrophages against Yersinia enterocolitica.
PG  - 489-95
LID - 10.1007/s12275-012-1534-6 [doi]
AB  - Nucleotide-binding oligomerization domain 2 (Nod2) is a cytosolic sensor for 
      muramyl dipeptide, a component of bacterial peptidoglycan. In this study, we have 
      examined whether Nod2 mediates the immune response of macrophages against 
      Yersinia enterocolitica. Bone-marrow-derived macrophages (BMDMs) were isolated 
      from WT and Nod2-deficient mice and were infected with various strains of Y. 
      enterocolitica. ELISA showed that the production of IL-6 and TNF-alpha in BMDMs 
      infected with Y. enterocolitica was not affected by the Nod2 deficiency. iNOS 
      mRNA expression was induced in both WT and Nod2-deficienct BMDMs in response to 
      Y. enterocolitica, beginning 2 h after infection. Nitric oxide (NO) production by 
      Y. enterocolitica did not differ between WT and Nod2-deficient BMDMs. Western 
      blot analysis revealed that Y. enterocolitica induces activation of NF-kappaB, p38, 
      and ERK MAPK through a Nod2-independent pathway. Neither LDH release by Y. 
      enterocolitica nor the phagocytic activity of the macrophages was altered by Nod2 
      deficiency. An in vivo experiment showed that bacterial clearance ability and 
      production of IL-6 and KC in serum were comparable in WT and Nod2-deficient mice 
      infected with Y. enterocolitica. These findings suggest that Nod2 may not be 
      critical for initiating the innate immune response of macrophages against 
      Yersinia infection.
FAU - Jeong, Yu-Jin
AU  - Jeong YJ
AD  - Department of Biochemistry, College of Medicine, Konyang University, Daejeon, 
      302-711, Republic of Korea.
FAU - Kim, Chang-Hwan
AU  - Kim CH
FAU - Song, Eun-Jung
AU  - Song EJ
FAU - Kang, Min-Jung
AU  - Kang MJ
FAU - Kim, Jee-Cheon
AU  - Kim JC
FAU - Oh, Sang-Muk
AU  - Oh SM
FAU - Lee, Kyung-Bok
AU  - Lee KB
FAU - Park, Jong-Hwan
AU  - Park JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120630
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Interleukin-6)
RN  - 0 (Nod2 Signaling Adaptor Protein)
RN  - 0 (Nod2 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - *Immunity, Innate
MH  - Interleukin-6/metabolism
MH  - Macrophages/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/biosynthesis
MH  - Nod2 Signaling Adaptor Protein/*deficiency
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Yersinia Infections/immunology/pathology
MH  - Yersinia enterocolitica/*immunology
EDAT- 2012/07/04 06:00
MHDA- 2012/10/30 06:00
CRDT- 2012/07/04 06:00
PHST- 2011/10/26 00:00 [received]
PHST- 2012/01/13 00:00 [accepted]
PHST- 2012/07/04 06:00 [entrez]
PHST- 2012/07/04 06:00 [pubmed]
PHST- 2012/10/30 06:00 [medline]
AID - 10.1007/s12275-012-1534-6 [doi]
PST - ppublish
SO  - J Microbiol. 2012 Jun;50(3):489-95. doi: 10.1007/s12275-012-1534-6. Epub 2012 Jun 
      30.