PMID- 22757777
OWN - NLM
STAT- MEDLINE
DCOM- 20121019
LR  - 20130606
IS  - 1365-2141 (Electronic)
IS  - 0007-1048 (Linking)
VI  - 158
IP  - 5
DP  - 2012 Sep
TI  - Human herpesvirus 6 positive Reed-Sternberg cells in nodular sclerosis Hodgkin 
      lymphoma.
PG  - 635-43
LID - 10.1111/j.1365-2141.2012.09206.x [doi]
AB  - Classical Hodgkin lymphoma (HL) exhibits a bi-modal age distribution that 
      suggests an infectious aetiology. However, most cases of nodular sclerosis HL 
      (NSHL) are Epstein-Barr virus (EBV) negative (60-90%). Previous studies regarding 
      human herpesvirus 6 (HHV-6) positivity of HL have led to conflicting results. In 
      order to clarify this situation, we examined NSHL biopsies for the presence and 
      distribution of HHV-6 by immunohistochemistry (IHC), polymerase chain reaction 
      (PCR), and fluorescence in situ hybridization (FISH). PCR identified HHV-6 DNA in 
      86% of NSHL cases. As HHV-6 DNA was also identified in most cases of reactive 
      lymphoid hyperplasia, we sought to localize the virus to specific cells by IHC, 
      which detected HHV-6 in Reed-Sternberg (RS) cells of nearly half (48%) of NSHL 
      cases. Dual CD30/HHV-6 immunostaining confirmed HHV-6 immunoreactivity in CD30+ 
      RS cells, and HHV-6 PCR positivity was confirmed in laser capture 
      microdissection-isolated CD30+ RS cells. FISH demonstrated multiple copies of 
      HHV-6 genome in scattered cells. In contrast, EBV+ RS cells were identified in 
      only 24% of the cases. HHV-6+ cases trended toward a younger age than EBV+ cases. 
      These results conclusively demonstrate that RS cells in many cases of NSHL are 
      HHV-6 positive, and suggest that HHV-6 may play a role in NSHL pathogenesis, 
      particularly in younger patients with EBV-negative disease.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Siddon, Alexa
AU  - Siddon A
AD  - Department of Pathology, Yale University School of Medicine, New Haven, CT 06520, 
      USA.
FAU - Lozovatsky, Larissa
AU  - Lozovatsky L
FAU - Mohamed, Ayman
AU  - Mohamed A
FAU - Hudnall, S David
AU  - Hudnall SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120704
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (DNA, Viral)
SB  - IM
CIN - Br J Haematol. 2013 May;161(4):594-5. PMID: 23406281
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - DNA, Viral/isolation & purification
MH  - Epstein-Barr Virus Infections/virology
MH  - Female
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Hodgkin Disease/*virology
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphoma, Follicular/virology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Reed-Sternberg Cells/*virology
MH  - Roseolovirus Infections/*virology
MH  - Sclerosis
MH  - Young Adult
EDAT- 2012/07/05 06:00
MHDA- 2012/10/20 06:00
CRDT- 2012/07/05 06:00
PHST- 2012/04/10 00:00 [received]
PHST- 2012/05/24 00:00 [accepted]
PHST- 2012/07/05 06:00 [entrez]
PHST- 2012/07/05 06:00 [pubmed]
PHST- 2012/10/20 06:00 [medline]
AID - 10.1111/j.1365-2141.2012.09206.x [doi]
PST - ppublish
SO  - Br J Haematol. 2012 Sep;158(5):635-43. doi: 10.1111/j.1365-2141.2012.09206.x. 
      Epub 2012 Jul 4.