PMID- 2275864
OWN - NLM
STAT- MEDLINE
DCOM- 19910305
LR  - 20181130
IS  - 0921-8912 (Print)
IS  - 0921-8912 (Linking)
VI  - 2
IP  - 3
DP  - 1990 Apr
TI  - The use of acridine orange cytofluorometry in the study of macrophage lysosomal 
      exocytosis.
PG  - 179-88
AB  - We present a rather simple cytofluorometric technique for the study of exocytosis 
      of lysosomal contents from individual cultured cells. It is based on the use of 
      the lysosomotropic weak base acridine orange (AO) which, in its stacked form, as 
      it occurs within lysosomes, emits red fluorescence when excited by blue light. 
      Mouse peritoneal macrophages were cultured for 48 h and, after 2 h in serum-free 
      medium, stained with AO. The cells were then exposed to F10-medium with or 
      without newborn calf serum (NCS), zymosan A (Z) or cytochalasin B (CB) for 
      different times at 20 or 37 degrees C. After staining, the macrophages showed no 
      change in red fluorescence intensity, if stored at room temperature in the dark. 
      If, however, the cells were kept in the incubator at 37 degrees C, the cells 
      showed slightly decreasing red fluorescence intensity with time. This decrease 
      was markedly potentiated by the presence of NCS, Z or CB, which are known to 
      induce secretion of lysosomal enzymes from macrophages in vitro. Selective 
      lysosomal enzyme release was confirmed biochemically during treatment with 
      zymosan A. The technique presented here may be of value in further studies on the 
      stimulation of, and the mechanisms behind, lysosomal exocytosis in cultured 
      cells.
FAU - Olsson, G M
AU  - Olsson GM
AD  - Department of Pathology, Faculty of Health Sciences, University of Linkoping, 
      Sweden.
FAU - Roberg, K
AU  - Roberg K
FAU - Rundquist, I
AU  - Rundquist I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Anal Cell Pathol
JT  - Analytical cellular pathology : the journal of the European Society for 
      Analytical Cellular Pathology
JID - 8911016
RN  - 0 (Culture Media)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 9010-72-4 (Zymosan)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Culture Media
MH  - Cytochalasin B/pharmacology
MH  - Exocytosis
MH  - Flow Cytometry
MH  - Lysosomes/drug effects/*metabolism
MH  - Macrophages/drug effects/*metabolism
MH  - Male
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Temperature
MH  - Zymosan/pharmacology
EDAT- 1990/04/01 00:00
MHDA- 1990/04/01 00:01
CRDT- 1990/04/01 00:00
PHST- 1990/04/01 00:00 [pubmed]
PHST- 1990/04/01 00:01 [medline]
PHST- 1990/04/01 00:00 [entrez]
PST - ppublish
SO  - Anal Cell Pathol. 1990 Apr;2(3):179-88.