PMID- 22759953
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 30
IP  - 1
DP  - 2012
TI  - Dendritic cells of synovium in experimental model of osteoarthritis of rabbits.
PG  - 23-32
LID - 10.1159/000339046 [doi]
AB  - AIMS: Dendritic cells (DCs) are critical initiators of immune responses, however, 
      its distribution and role in osteoarthritis (OA) remains largely unknown. This 
      study is to investigate the distribution of DCs in the rabbits' synovium of 
      experimental OA. METHODS: Model of OA was established by excising the medial 
      meniscus of both hind knees in New Zealand white rabbits. The grades of synovium 
      and articular cartilage were assessed and scored by hematoxylin eosin stain after 
      2, 4, 8, and 12 weeks of operation. The distribution of DCs was investigated by 
      immunohistochemistry staining in the synovium from OA rabbits. The levels of 
      IL-1beta and TNF-alpha in synovial fluid were measured by ELISA kits. RESULTS: Molecular 
      markers for DCs, such as DC-LAMP, CD80, CD83, and CD86 were detected in lymphoid 
      aggregations and perivenular infiltration areas in the synovium from OA rabbits. 
      Large numbers of DCs were observed in the synovium in the early stages (2 or 4 
      weeks) after operation. The number of DCs was significantly increased with the 
      progression of inflammatory grade in synovium in the same early stages. 
      Expression of IL-1beta and TNF-alpha were also increased in the early stages, then 
      decreased with the inflammatory regression in synovium. CONCLUSION: The data from 
      this study strongly suggested that DCs may play a key role, at least in part, in 
      inflammation of the OA pathogenesis, especially in the early stages of OA.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - E, Xiaoqiang
AU  - E X
AD  - Department of Orthopedics, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Cao, Yang
AU  - Cao Y
FAU - Meng, Hongxue
AU  - Meng H
FAU - Qi, Yuebin
AU  - Qi Y
FAU - Du, Guangye
AU  - Du G
FAU - Xu, Jun
AU  - Xu J
FAU - Bi, Zhenggang
AU  - Bi Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120705
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antigens, CD)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Cartilage, Articular/pathology
MH  - Cell Count
MH  - Dendritic Cells/*metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Femur/pathology
MH  - Interleukin-1beta/metabolism
MH  - Male
MH  - Osteoarthritis, Knee/metabolism/*pathology
MH  - Rabbits
MH  - Synovial Fluid/metabolism
MH  - Synovial Membrane/metabolism/*pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2012/07/05 06:00
MHDA- 2012/10/23 06:00
CRDT- 2012/07/05 06:00
PHST- 2012/04/24 00:00 [accepted]
PHST- 2012/07/05 06:00 [entrez]
PHST- 2012/07/05 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
AID - 000339046 [pii]
AID - 10.1159/000339046 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2012;30(1):23-32. doi: 10.1159/000339046. Epub 2012 Jul 5.