PMID- 22759969
OWN - NLM
STAT- MEDLINE
DCOM- 20121022
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 30
IP  - 1
DP  - 2012
TI  - Store-operated Ca2+ entry (SOCE) plays a role in the polarization of 
      neutrophil-like HL-60 cells by regulating the activation of Akt, Src, and Rho 
      family GTPases.
PG  - 221-37
LID - 10.1159/000339059 [doi]
AB  - Neutrophil polarization is a basic activity involved in the innate immune 
      response, and it may be initiated by extracellular Ca(2+) entry, a process 
      primarily mediated through store-operated Ca(2+) entry (SOCE). Yet, the 
      mechanisms by which SOCE participates in cell polarization remain unclear. We 
      hypothesized that Akt- and Src-dependent pathways, traditionally linked to 
      neutrophil polarization, may interact with SOCE in this event. In this study, 
      SKF96365 and 2-APB, inhibitors of SOCE as proved by their inhibition on Mn(2+) 
      influx, were observed to inhibit the formyl-methionyl-leucyl-phenylalanine 
      (fMLP)-induced influx of Ca(2+), the activation of Akt, Src, Rac1, Rac2, and 
      Cdc42, and the polarization of differentiated HL-60 (dHL-60) cells. 
      Downregulation of stromal interaction molecule 1 (STIM1), a Ca(2+) sensor 
      identified to induce SOCE, by siRNA led to decreases in the following indexes: 
      Ca(2+) entry, activation of Akt, Src, Rac2 (rather than Rac1) and Cdc42, and 
      fMLP-induced polarization. This study suggests that SOCE might be the predominant 
      form of Ca(2+) entry involved in the regulation of cell polarization, and it may 
      act through the Akt/Src/Rac pathways, as modeled in dHL-60 cells. It also 
      suggests that STIM1 is a key modulator of cell polarization, potentially serving 
      as a target for the designation of anti-immune deficiency therapies.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Zou, Wenying
AU  - Zou W
AD  - Department of Occupational Health and Occupational Medicine, School of Public 
      Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong, 
      China.
FAU - Meng, Xiaojing
AU  - Meng X
FAU - Cai, Chunqing
AU  - Cai C
FAU - Zou, Mengchen
AU  - Zou M
FAU - Tang, Shihao
AU  - Tang S
FAU - Chu, Xinwei
AU  - Chu X
FAU - Wang, Xubu
AU  - Wang X
FAU - Zou, Fei
AU  - Zou F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120619
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Boron Compounds)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Imidazoles)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (STIM1 protein, human)
RN  - 0 (Stromal Interaction Molecule 1)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - E4ES684O93 (2-aminoethoxydiphenyl borate)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - I61V87164A (1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole)
SB  - IM
MH  - Boron Compounds/pharmacology
MH  - Calcium Channel Blockers/pharmacology
MH  - *Calcium Signaling
MH  - *Cell Polarity
MH  - *Enzyme Activation
MH  - Gene Knockdown Techniques
MH  - HL-60 Cells
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Manganese/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neoplasm Proteins/genetics/metabolism
MH  - Neutrophils/*metabolism/physiology
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - RNA Interference
MH  - Stromal Interaction Molecule 1
MH  - rho GTP-Binding Proteins/*metabolism
MH  - src-Family Kinases/*metabolism
EDAT- 2012/07/05 06:00
MHDA- 2012/10/23 06:00
CRDT- 2012/07/05 06:00
PHST- 2012/05/14 00:00 [accepted]
PHST- 2012/07/05 06:00 [entrez]
PHST- 2012/07/05 06:00 [pubmed]
PHST- 2012/10/23 06:00 [medline]
AID - 000339059 [pii]
AID - 10.1159/000339059 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2012;30(1):221-37. doi: 10.1159/000339059. Epub 2012 Jun 
      19.