PMID- 22761300
OWN - NLM
STAT- MEDLINE
DCOM- 20130115
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 19
IP  - 9
DP  - 2012 Sep
TI  - Immunostimulatory effects of recombinant Erysipelothrix rhusiopathiae expressing 
      porcine interleukin-18 in mice and pigs.
PG  - 1393-8
LID - 10.1128/CVI.00342-12 [doi]
AB  - Interleukin-18 (IL-18), which was originally called gamma interferon 
      (IFN-gamma)-inducing factor, has been shown to play an important role in innate and 
      acquired immune responses. In this study, attenuated Erysipelothrix rhusiopathiae 
      strains were engineered to produce porcine IL-18 (poIL-18) and evaluated for 
      their potential immunostimulatory effect in animals. Recombinant poIL-18 was 
      successfully expressed in the recombinant E. rhusiopathiae strains YS-1/IL-18 and 
      KO/IL-18. The culture supernatant of YS-1/IL-18 was confirmed to induce IFN-gamma 
      production in murine splenocytes in vitro, and this production was inhibited by 
      incubation with anti-poIL-18 monoclonal antibodies. Furthermore, more IFN-gamma 
      production was induced upon stimulation of splenocytes with concanavalin A for 
      splenocytes from mice that were intraperitoneally inoculated with YS-1/IL-18 than 
      for splenocytes from control mice inoculated with the parent strain YS-1. 
      Peritoneal macrophages from mice preinoculated with YS-1/IL-18 exhibited enhanced 
      phagocytosis of Salmonella enterica subsp. enterica serovar Typhimurium compared 
      with peritoneal macrophages from control mice preinoculated with YS-1. We also 
      confirmed the immunostimulatory effect on humoral immune responses against 
      antigens of E. rhusiopathiae and Mycoplasma hyopneumoniae in gnotobiotic pigs 
      that were orally preinoculated with KO/IL-18. Thus, these results provide 
      evidence that E. rhusiopathiae is a promising vector for the expression of host 
      cytokines and suggest the potential utility of E. rhusiopathiae vector-encoded 
      cytokines in the activation of host innate and acquired immune responses.
FAU - Ogawa, Yohsuke
AU  - Ogawa Y
AD  - National Institute of Animal Health, National Agriculture and Food Research 
      Organization, Tsukuba, Ibaraki, Japan.
FAU - Minagawa, Yu
AU  - Minagawa Y
FAU - Shi, Fang
AU  - Shi F
FAU - Eguchi, Masahiro
AU  - Eguchi M
FAU - Muneta, Yoshihiro
AU  - Muneta Y
FAU - Shimoji, Yoshihiro
AU  - Shimoji Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120703
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Interleukin-18)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Synthetic)
SB  - IM
MH  - Animals
MH  - Bacterial Vaccines/administration & dosage/genetics/*immunology
MH  - Erysipelothrix/genetics/*immunology
MH  - Female
MH  - Interleukin-18/genetics/*immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Macrophages, Peritoneal/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phagocytosis
MH  - Spleen/immunology
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/genetics/immunology
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology
PMC - PMC3428390
EDAT- 2012/07/05 06:00
MHDA- 2013/01/16 06:00
PMCR- 2013/03/01
CRDT- 2012/07/05 06:00
PHST- 2012/07/05 06:00 [entrez]
PHST- 2012/07/05 06:00 [pubmed]
PHST- 2013/01/16 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - CVI.00342-12 [pii]
AID - 00342-12 [pii]
AID - 10.1128/CVI.00342-12 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2012 Sep;19(9):1393-8. doi: 10.1128/CVI.00342-12. Epub 2012 
      Jul 3.