PMID- 22761932
OWN - NLM
STAT- MEDLINE
DCOM- 20130104
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 6
DP  - 2012
TI  - Learning and nicotine interact to increase CREB phosphorylation at the jnk1 
      promoter in the hippocampus.
PG  - e39939
LID - 10.1371/journal.pone.0039939 [doi]
LID - e39939
AB  - Nicotine is known to enhance long-term hippocampus dependent learning and memory 
      in both rodents and humans via its activity at nicotinic acetylcholinergic 
      receptors (nAChRs). However, the molecular basis for the nicotinic modulation of 
      learning is incompletely understood. Both the mitogen activated protein kinases 
      (MAPKs) and cAMP response element binding protein (CREB) are known to be integral 
      to the consolidation of long-term memory and the disruption of MAPKs and CREB are 
      known to abrogate some of the cognitive effects of nicotine. In addition, the 
      acquisition of contextual fear conditioning in the presence of nicotine is 
      associated with a beta2-subunit containing nAChR-dependent increase in jnk1 (mapk8) 
      transcription in the hippocampus. In the present study, chromatin 
      immunoprecipitation (ChIP) was used to examine whether learning and nicotine 
      interact to alter transcription factor binding or histone acetylation at the jnk1 
      promoter region. The acquisition of contextual fear conditioning in the presence 
      of nicotine resulted in an increase in phosphorylated CREB (pCREB) binding to the 
      jnk1 promoter in the hippocampus in a beta2-subunit containing nAChR dependent 
      manner, but had no effect on CREB binding; neither fear conditioning alone nor 
      nicotine administration alone altered transcription factor binding to the jnk1 
      promoter. In addition, there were no changes in histone H3 or H4 acetylation at 
      the jnk1 promoter following fear conditioning in the presence of nicotine. These 
      results suggest that contextual fear learning and nicotine administration act 
      synergistically to produce a unique pattern of protein activation and gene 
      transcription in the hippocampus that is not individually generated by fear 
      conditioning or nicotine administration alone.
FAU - Kenney, Justin W
AU  - Kenney JW
AD  - Department of Psychology, Temple University, Philadelphia, Pennsylvania, United 
      States of America.
FAU - Poole, Rachel L
AU  - Poole RL
FAU - Adoff, Michael D
AU  - Adoff MD
FAU - Logue, Sheree F
AU  - Logue SF
FAU - Gould, Thomas J
AU  - Gould TJ
LA  - eng
GR  - R01 DA024787/DA/NIDA NIH HHS/United States
GR  - P50 CA143187/CA/NCI NIH HHS/United States
GR  - DA024787/DA/NIDA NIH HHS/United States
GR  - T32 DA007237/DA/NIDA NIH HHS/United States
GR  - DA017949/DA/NIDA NIH HHS/United States
GR  - R01 DA017949/DA/NIDA NIH HHS/United States
GR  - DA07237/DA/NIDA NIH HHS/United States
GR  - CA143187/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20120628
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA Primers)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
SB  - IM
MH  - Base Sequence
MH  - Chromatin Immunoprecipitation
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - DNA Primers
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fear
MH  - Hippocampus/*drug effects/metabolism/physiology
MH  - *Learning
MH  - Mitogen-Activated Protein Kinase 8/*genetics
MH  - Nicotine/*pharmacology
MH  - Phosphorylation
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
PMC - PMC3386232
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/07/05 06:00
MHDA- 2013/01/05 06:00
PMCR- 2012/06/28
CRDT- 2012/07/05 06:00
PHST- 2012/04/02 00:00 [received]
PHST- 2012/05/29 00:00 [accepted]
PHST- 2012/07/05 06:00 [entrez]
PHST- 2012/07/05 06:00 [pubmed]
PHST- 2013/01/05 06:00 [medline]
PHST- 2012/06/28 00:00 [pmc-release]
AID - PONE-D-12-09185 [pii]
AID - 10.1371/journal.pone.0039939 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(6):e39939. doi: 10.1371/journal.pone.0039939. Epub 2012 Jun 28.