PMID- 22762447
OWN - NLM
STAT- MEDLINE
DCOM- 20140508
LR  - 20190318
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Linking)
VI  - 60
IP  - 4
DP  - 2013 Aug
TI  - Pathogenicity and distribution of highly pathogenic porcine reproductive and 
      respiratory syndrome virus in pigs.
PG  - 351-9
LID - 10.1111/j.1865-1682.2012.01354.x [doi]
AB  - The pathogenesis of highly pathogenic porcine reproductive and respiratory 
      syndrome virus (PRRSV) strain (HuN4) is poorly understood. Therefore, highly 
      pathogenic PRRSV strain (HuN4) and its derivative strain (HuN4-F112) (obtained by 
      propagation in MARC145 cells for 112 passages) were inoculated into a total of 48 
      PRRSV-sero-negative pigs (age: 4-5 weeks) by the intranasal route. Virological, 
      pathological and in situ hybridization analyses were performed. The results 
      exhibited that pigs infected with HuN4 showed a loss of appetite, decrease in 
      body weight, raised body temperature and respiratory symptoms, along with 
      interstitial pneumonia lesions. In the HuN4 group, multifocal interstitial 
      pneumonia with macrophage infiltration was found in the lung. The lesions in the 
      lymph node were characterized by collapsed follicles, depletion of germinal 
      centres and reduction in lymphocytes. Perivascular cuffing and glial nodules were 
      observed in the brains of some pigs. By comparison, the HuN4-F112 group had 
      milder lesions. PRRSV was detected in macrophages, alveolar epithelial cells and 
      vascular endothelial cells in the tonsil and lymph nodes. The PRRSV amounts in 
      the pigs infected with HuN4 were 10(5) -10(9) copies/ml in the blood and 10(10) 
      -10(11) copies/g in the lung tissues, whereas the virus amounts with HuN4-F112 
      were 10(2.15) -10(3.13) copies/ml in the blood and 10(3.0) -10(3.6) copies/g in 
      the lung. Our results demonstrate that the PRRS HuN4 virus infects alveolar 
      epithelial cells, macrophages and vascular endothelial cells causing diffuse 
      alveolar damage and lymph node necrosis. Its higher pathogenicity compared with 
      HuN4-F112 virus may be explained in part by higher replication rate in the 
      previously mentioned organs.
CI  - (c) 2012 Blackwell Verlag GmbH.
FAU - Hu, S P
AU  - Hu SP
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Zhang, Z
AU  - Zhang Z
FAU - Liu, Y G
AU  - Liu YG
FAU - Tian, Z J
AU  - Tian ZJ
FAU - Wu, D L
AU  - Wu DL
FAU - Cai, X H
AU  - Cai XH
FAU - He, X J
AU  - He XJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120705
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Brain/pathology/virology
MH  - Endothelium, Vascular/pathology/*virology
MH  - In Situ Hybridization
MH  - Lung/pathology/*virology
MH  - Lymph Nodes/pathology/*virology
MH  - Macrophages/pathology/*virology
MH  - Palatine Tonsil/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*pathology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/isolation & 
      purification/*pathogenicity
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Swine
MH  - Virulence
MH  - Virus Replication
OTO - NOTNLM
OT  - HP-PRRSV
OT  - in situ hybridization
OT  - pathology
OT  - virology
EDAT- 2012/07/06 06:00
MHDA- 2014/05/09 06:00
CRDT- 2012/07/06 06:00
PHST- 2012/07/06 06:00 [entrez]
PHST- 2012/07/06 06:00 [pubmed]
PHST- 2014/05/09 06:00 [medline]
AID - 10.1111/j.1865-1682.2012.01354.x [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2013 Aug;60(4):351-9. doi: 
      10.1111/j.1865-1682.2012.01354.x. Epub 2012 Jul 5.