PMID- 22762953
OWN - NLM
STAT- MEDLINE
DCOM- 20121113
LR  - 20220311
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1252
DP  - 2012 Aug 24
TI  - Repeatability of the efficiency of columns packed with sub-3mum core-shell 
      particles: Part I. 2.6mum Kinetex-C(18) particles in 4.6mm and 2.1mmx100mm column 
      formats.
PG  - 31-44
LID - 10.1016/j.chroma.2012.05.072 [doi]
AB  - The column-to-column repeatability of the mass transfer mechanism in columns 
      packed with sub-3mum shell particles was investigated. The parameters of this 
      mechanism were measured for twelve columns (six 2.1mmx100mm and six 4.6mmx100mm) 
      packed with the same batch of 2.6mum Kinetex-C(18) particles (Phenomenex, CA, 
      USA). For both series, the manufacturer provided columns at different positions 
      in the efficiency distribution given by the quality test control. Three compounds 
      were used, uracil, naphthalene and insulin. The reduced longitudinal diffusion 
      term was measured with the peak parking (PP) method, the reduced solid-liquid 
      mass transfer resistance term was given by a combination of the PP results and a 
      model of effective diffusion in ternary composite materials (non-porous cores, 
      concentric porous shell, and eluent matrix), validated previously. The overall 
      eddy diffusion term was obtained by subtraction of these two HETP terms from the 
      overall reduced HETP measured by numerical integration of the entire peak 
      profiles. The results demonstrate that the dispersion of the column efficiencies 
      is only due to the random nature of the packing process. At the highest reduced 
      velocity achieved, the relative standard deviations (RSDs) of the eddy diffusion 
      term for the 2.1mm I.D. columns were ca. 7% and 3% for the low molecular weight 
      compounds and for insulin, respectively. For the 4.6mm I.D. columns, these RSDs 
      were 15% and 5%, respectively. The larger RSDs for the 4.6mm I.D. columns is 
      explained by the exceptionally low value of the eddy diffusion term.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Gritti, Fabrice
AU  - Gritti F
AD  - Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
FAU - Guiochon, Georges
AU  - Guiochon G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120615
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Insulin)
RN  - 0 (Naphthalenes)
RN  - 2166IN72UN (naphthalene)
RN  - 56HH86ZVCT (Uracil)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*instrumentation
MH  - Diffusion
MH  - Insulin/chemistry
MH  - *Microspheres
MH  - Models, Chemical
MH  - Molecular Weight
MH  - Naphthalenes/chemistry
MH  - Permeability
MH  - Porosity
MH  - Reproducibility of Results
MH  - Temperature
MH  - Uracil/chemistry
EDAT- 2012/07/06 06:00
MHDA- 2012/11/14 06:00
CRDT- 2012/07/06 06:00
PHST- 2012/04/11 00:00 [received]
PHST- 2012/05/17 00:00 [revised]
PHST- 2012/05/21 00:00 [accepted]
PHST- 2012/07/06 06:00 [entrez]
PHST- 2012/07/06 06:00 [pubmed]
PHST- 2012/11/14 06:00 [medline]
AID - S0021-9673(12)00804-7 [pii]
AID - 10.1016/j.chroma.2012.05.072 [doi]
PST - ppublish
SO  - J Chromatogr A. 2012 Aug 24;1252:31-44. doi: 10.1016/j.chroma.2012.05.072. Epub 
      2012 Jun 15.