PMID- 22764015
OWN - NLM
STAT- MEDLINE
DCOM- 20121128
LR  - 20120718
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 137
IP  - 16
DP  - 2012 Aug 21
TI  - Sampling and characterisation of volatile organic compound profiles in human 
      saliva using a polydimethylsiloxane coupon placed within the oral cavity.
PG  - 3627-34
LID - 10.1039/c2an35432b [doi]
AB  - Evaluation of published methods reveals that existing methods for saliva sampling 
      do not address the physical-chemical attributes of volatile organic compounds 
      (VOCs). This study describes and presents evidence for adopting in situ sampling 
      of salivary VOCs directly from the oral cavity using a polydimethylsiloxane 
      (PDMS) based sampler. In vitro studies indicated that the vapour pressure of 
      analytes was a factor in both the recovery of analytes and the precision of the 
      recovery. The highest recoveries were observed for VOCs with the lowest vapour 
      pressures, for example 5-nonanol (vapour pressure (P(v)) = 14 Pa) recoveries were 
      approximately 20 times greater than those observed for octane (P(v) = 1726 Pa). 
      Similarly, relative standard deviations reduced with vapour pressure, with the 
      RSD for 5-nonanol responses observed to be 2.7% when compared to RSD = 26% for 
      octane. Evaluation of VOCs recovered from 6 in vivo samples indicated that VOC 
      concentrations in saliva may follow log-normal distributions; log-normal RSDs 
      falling between 4.4% and 18.2% across the range of volatilities encountered. 
      Increasing sampling time from 1 to 30 minutes indicated that the recovery of VOC 
      into the sampler was affected by interaction between different physical-chemical 
      properties and biogenic flux. A sampling time of 10 min was found to offer an 
      acceptable compromise that enabled a representative sample to be acquired for the 
      widest range of observed VOC behaviours with the sampler. The potential to 'tune' 
      the sampling protocol for targeted analysis based on these factors was also 
      noted. Comparison with passive drool saliva collection revealed up to 10(5) 
      enhancement with reduced variability compared to drooled samples. This approach 
      to in situ saliva sampling appears to have significant analytical utility for 
      studying volatile signatures in humans.
FAU - Martin, Helen J
AU  - Martin HJ
AD  - Centre for Analytical Science, Department of Chemistry, Loughborough University, 
      Loughborough, Leicestershire, LE11 3TU, UK.
FAU - Riazanskaia, Svetlana
AU  - Riazanskaia S
FAU - Thomas, C L Paul
AU  - Thomas CL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120704
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Volatile Organic Compounds)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Clinical Chemistry Tests/*methods
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Male
MH  - Mouth/*chemistry
MH  - Reproducibility of Results
MH  - Saliva/*chemistry
MH  - Sialorrhea
MH  - Specimen Handling/*methods
MH  - Time Factors
MH  - Volatile Organic Compounds/*analysis
MH  - Young Adult
EDAT- 2012/07/06 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/07/06 06:00
PHST- 2012/07/06 06:00 [entrez]
PHST- 2012/07/06 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1039/c2an35432b [doi]
PST - ppublish
SO  - Analyst. 2012 Aug 21;137(16):3627-34. doi: 10.1039/c2an35432b. Epub 2012 Jul 4.