PMID- 22768284
OWN - NLM
STAT- MEDLINE
DCOM- 20121127
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 6
DP  - 2012
TI  - Longitudinal in vivo imaging of retinal ganglion cells and retinal thickness 
      changes following optic nerve injury in mice.
PG  - e40352
LID - 10.1371/journal.pone.0040352 [doi]
LID - e40352
AB  - BACKGROUND: Retinal ganglion cells (RGCs) die in sight-threatening eye diseases. 
      Imaging RGCs in humans is not currently possible and proof of principle in 
      experimental models is fundamental for future development. Our objective was to 
      quantify RGC density and retinal thickness following optic nerve transection in 
      transgenic mice expressing cyan fluorescent protein (CFP) under control of the 
      Thy1 promoter, expressed by RGCs and other neurons. METHODOLOGY/PRINCIPAL 
      FINDINGS: A modified confocal scanning laser ophthalmoscopy 
      (CSLO)/spectral-domain optical coherence tomography (SD-OCT) camera was used to 
      image and quantify CFP+ cells in mice from the B6.Cg-Tg(Thy1-CFP)23Jrs/J line. 
      SD-OCT circle (1 B-scan), raster (37 B-scans) and radial (24 B-scans) scans of 
      the retina were also obtained. CSLO was performed at baseline (n = 11) and 3 
      (n = 11), 5 (n = 4), 7 (n = 10), 10 (n = 6), 14 (n = 7) and 21 (n = 5) days 
      post-transection, while SD-OCT was performed at baseline and 7, 14 and 35 days 
      (n = 9) post-transection. Longitudinal change in CFP+ cell density and retinal 
      thickness were computed. Compared to baseline, the mean (SD) percentage CFP+ 
      cells remaining at 3, 5, 7, 10, 14 and 21 days post-transection was 86 (9)%, 63 
      (11)%, 45 (11)%, 31 (9)%, 20 (9)% and 8 (4)%, respectively. Compared to baseline, 
      the mean (SD) retinal thickness at 7 days post-transection was 97 (3)%, 98 (2)% 
      and 97 (4)% for the circle, raster and radial scans, respectively. The 
      corresponding figures at 14 and 35 days post-transection were 96 (3)%, 97 (2)% 
      and 95 (3)%; and 93 (3)%, 94 (3)% and 92 (3)%. CONCLUSIONS/SIGNIFICANCE: 
      Longitudinal imaging showed an exponential decline in CFP+ cell density and a 
      small (</=8%) reduction in SD-OCT measured retinal thickness post-transection. 
      SD-OCT is a promising tool for detecting structural changes in experimental optic 
      neuropathy. These results represent an important step towards translation for 
      clinical use.
FAU - Chauhan, Balwantray C
AU  - Chauhan BC
AD  - Retina and Optic Nerve Research Laboratory, Dalhousie University, Halifax, Nova 
      Scotia, Canada. bal@dal.ca
FAU - Stevens, Kelly T
AU  - Stevens KT
FAU - Levesque, Julie M
AU  - Levesque JM
FAU - Nuschke, Andrea C
AU  - Nuschke AC
FAU - Sharpe, Glen P
AU  - Sharpe GP
FAU - O'Leary, Neil
AU  - O'Leary N
FAU - Archibald, Michele L
AU  - Archibald ML
FAU - Wang, Xu
AU  - Wang X
LA  - eng
GR  - MOP-89808/Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120629
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cyan Fluorescent Protein)
RN  - 0 (Thy-1 Antigens)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Green Fluorescent Proteins/metabolism
MH  - Mice
MH  - Ophthalmoscopy/*methods
MH  - Optic Nerve Injuries/*pathology
MH  - Promoter Regions, Genetic/genetics
MH  - Retinal Ganglion Cells/*pathology
MH  - Thy-1 Antigens/genetics
MH  - Tomography, Optical Coherence/*methods
PMC - PMC3386976
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2012/07/07 06:00
MHDA- 2012/12/10 06:00
PMCR- 2012/06/29
CRDT- 2012/07/07 06:00
PHST- 2011/08/11 00:00 [received]
PHST- 2012/06/07 00:00 [accepted]
PHST- 2012/07/07 06:00 [entrez]
PHST- 2012/07/07 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2012/06/29 00:00 [pmc-release]
AID - PONE-D-11-15814 [pii]
AID - 10.1371/journal.pone.0040352 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(6):e40352. doi: 10.1371/journal.pone.0040352. Epub 2012 Jun 29.