PMID- 22768859
OWN - NLM
STAT- MEDLINE
DCOM- 20121107
LR  - 20181201
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 28
IP  - 7
DP  - 2012 Jul
TI  - [Effects of megsin gene transfection on the expressions of MCP-1 and ICAM-1 in 
      mouse glomerular mesangial cells].
PG  - 702-5
AB  - AIM: To observe the effects of megsin gene transfection on the expressions of 
      monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion 
      molecule-1(ICAM-1). METHODS: Mouse glomerular mesangial cells were cultured in 
      high glucose medium, and then cell proliferation was measured by MTT assay at 12, 
      24 and 48 h respectively. The expressions of megsin, MCP-1 and ICAM-1 in 
      mesangial cells were detected by immunocytochemical staining and Western 
      blotting. The concentration of type IV collagen in the culture supernatant of 
      mesangial cells was measured by ELISA. RESULTS: Under high glucose concentration, 
      the expressions of megsin, MCP-1 and ICAM-1 increased; the concentration of type 
      IV collagen in the cell supernatant was elevated as well; and mesangial cell 
      proliferation was enhanced. After megsin gene transfection, the above changes 
      were more significant, but were abated following megsin shRNA/transfection. 
      CONCLUSION: Megsin gene can up-regulate the expressions of MCP-1 and ICAM-1, 
      promote mesangial cell proliferation and mesangial extracellular matrix 
      accumulation.
FAU - Liu, Jie
AU  - Liu J
AD  - Department of Nephrology, Third Hospital of Hebei Medical University, 
      Shijiazhuang 050051, China. gwliujie@126.com
FAU - Li, Ying
AU  - Li Y
FAU - Liu, Mao-dong
AU  - Liu MD
FAU - Ren, Guang-wei
AU  - Ren GW
FAU - Wang, Ming-ming
AU  - Wang MM
FAU - Li, Ying-min
AU  - Li YM
FAU - Cong, Bin
AU  - Cong B
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Collagen Type IV)
RN  - 0 (Serpinb7 protein, mouse)
RN  - 0 (Serpins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Proliferation
MH  - Chemokine CCL2/*metabolism
MH  - Collagen Type IV/metabolism
MH  - *Gene Expression Regulation
MH  - Glomerular Mesangium/metabolism
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Mesangial Cells/*metabolism
MH  - Mice
MH  - Serpins/*genetics/*metabolism
MH  - Transfection
EDAT- 2012/07/10 06:00
MHDA- 2012/11/08 06:00
CRDT- 2012/07/10 06:00
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2012/11/08 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Jul;28(7):702-5.