PMID- 22770505
OWN - NLM
STAT- MEDLINE
DCOM- 20130306
LR  - 20181201
IS  - 1543-8392 (Electronic)
IS  - 1543-8384 (Linking)
VI  - 9
IP  - 9
DP  - 2012 Sep 4
TI  - Interactions of liposome carriers with infectious fungal hyphae reveals the role 
      of beta-glucans.
PG  - 2489-96
LID - 10.1021/mp300097k [doi]
AB  - Relatively little is known about how liposomal formulations modulate drug 
      delivery to fungal pathogens. We compared patterns of hyphal cell wall binding 
      for empty rhodmine-labeled liposomes and the clinically available amphotericin 
      B-containing liposomal formulation (AmBisome) in Aspergillus fumigatus and 
      Candida albicans. Following 0.5 h of coincubation with A. fumigatus , empty 
      liposomes concentrated primarily in fungal septae along at the surface of the 
      cell wall, suggesting that liposome uptake is concentrated in areas of the cell 
      wall where linear glucan is exposed on the cell surface, which was confirmed by 
      aniline blue staining. Consistent with this hypothesis, pretreatment of liposomes 
      with soluble linear glucan (laminarin) decreased liposome binding in both 
      Aspergillus and Candida fungal hyphae, while growth of Aspergillus hyphae in the 
      presence of an agent that increases fungal cell wall surface exposure of linear 
      beta-glucans without cell death (caspofungin) increased liposome uptake throughout 
      the Aspergillus fungal cell wall. Increasing the polyethylene glycol (PEG) 
      concentration in liposomes from 0 to 30% significantly increased fungal uptake of 
      liposomes that was only modestly attenuated when fungal cells were incubated in 
      serum concentrations ranging from 10 to 100%. The presence of beta-glucans on the 
      fungal hyphae cell walls of Aspergillus fumigatus is one of the factors 
      responsible for mediating the binding of liposome carriers to the hyphae and 
      could explain possible synergy reported between liposomal amphotericin B and 
      echinocanins.
FAU - Chavan, Neelam L
AU  - Chavan NL
AD  - Department of Pharmacological & Pharmaceutical Sciences, College of Pharmacy, 
      University of Houston, Houston, Texas 77030, United States.
FAU - Young, Joseph K
AU  - Young JK
FAU - Drezek, Rebekah A
AU  - Drezek RA
FAU - Lewis, Russell
AU  - Lewis R
FAU - Bikram, Malavosklish
AU  - Bikram M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120802
PL  - United States
TA  - Mol Pharm
JT  - Molecular pharmaceutics
JID - 101197791
RN  - 0 (Aniline Compounds)
RN  - 0 (Antifungal Agents)
RN  - 0 (Drug Carriers)
RN  - 0 (Echinocandins)
RN  - 0 (Glucans)
RN  - 0 (Lipopeptides)
RN  - 0 (Liposomes)
RN  - 0 (Polysaccharides)
RN  - 0 (beta-Glucans)
RN  - 0 (liposomal amphotericin B)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 7XU7A7DROE (Amphotericin B)
RN  - 8004-91-9 (aniline blue)
RN  - 9008-22-4 (laminaran)
RN  - F0XDI6ZL63 (Caspofungin)
SB  - IM
MH  - Amphotericin B/*pharmacokinetics/pharmacology
MH  - Aniline Compounds/pharmacology
MH  - Antifungal Agents/pharmacokinetics/pharmacology
MH  - Aspergillus fumigatus/drug effects/*metabolism
MH  - Candida albicans/drug effects/*metabolism
MH  - Caspofungin
MH  - Cell Wall/drug effects/metabolism
MH  - Chemistry, Pharmaceutical/methods
MH  - Drug Carriers/*pharmacokinetics
MH  - Echinocandins/pharmacology
MH  - Glucans
MH  - Hyphae/drug effects/*metabolism
MH  - Lipopeptides
MH  - Liposomes/*pharmacokinetics
MH  - Models, Molecular
MH  - Polyethylene Glycols/chemistry
MH  - Polysaccharides/pharmacology
MH  - beta-Glucans/*metabolism
EDAT- 2012/07/10 06:00
MHDA- 2013/03/07 06:00
CRDT- 2012/07/10 06:00
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2013/03/07 06:00 [medline]
AID - 10.1021/mp300097k [doi]
PST - ppublish
SO  - Mol Pharm. 2012 Sep 4;9(9):2489-96. doi: 10.1021/mp300097k. Epub 2012 Aug 2.