PMID- 22770567
OWN - NLM
STAT- MEDLINE
DCOM- 20121126
LR  - 20211021
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 33
IP  - 28
DP  - 2012 Oct
TI  - In vivo targeting of alveolar macrophages via RAFT-based glycopolymers.
PG  - 6889-97
LID - 10.1016/j.biomaterials.2012.06.025 [doi]
AB  - Targeting cell populations via endogenous carbohydrate receptors is an appealing 
      approach for drug delivery. However, to be effective, this strategy requires the 
      production of high affinity carbohydrate ligands capable of engaging with 
      specific cell-surface lectins. To develop materials that exhibit high affinity 
      towards these receptors, we synthesized glycopolymers displaying pendent 
      carbohydrate moieties from carbohydrate-functionalized monomer precursors via 
      reversible addition-fragmentation chain transfer (RAFT) polymerization. These 
      glycopolymers were fluorescently labeled and used to determine 
      macrophage-specific targeting both in vitro and in vivo. Mannose- and 
      N-acetylglucosamine-containing glycopolymers were shown to specifically target 
      mouse bone marrow-derived macrophages (BMDMs) in vitro in a dose-dependent manner 
      as compared to a galactose-containing glycopolymer (30- and 19-fold higher 
      uptake, respectively). In addition, upon macrophage differentiation, the mannose 
      glycopolymer exhibited enhanced uptake in M2-polarized macrophages, an 
      anti-inflammatory macrophage phenotype prevalent in injured tissue. This 
      carbohydrate-specific uptake was retained in vivo, as alveolar macrophages 
      demonstrated 6-fold higher internalization of mannose glycopolymer, as compared 
      to galactose, following intratracheal administration in mice. We have shown the 
      successful synthesis of a class of functional RAFT glycopolymers capable of 
      macrophage-type specific uptake both in vitro and in vivo, with significant 
      implications for the design of future targeted drug delivery systems.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Song, Eun-Ho
AU  - Song EH
AD  - Department of Bioengineering, University of Washington, Seattle, WA 98195, USA. 
      ehsong74@uw.edu
FAU - Manganiello, Matthew J
AU  - Manganiello MJ
FAU - Chow, Yu-Hua
AU  - Chow YH
FAU - Ghosn, Bilal
AU  - Ghosn B
FAU - Convertine, Anthony J
AU  - Convertine AJ
FAU - Stayton, Partick S
AU  - Stayton PS
FAU - Schnapp, Lynn M
AU  - Schnapp LM
FAU - Ratner, Daniel M
AU  - Ratner DM
LA  - eng
GR  - K24 HL086796/HL/NHLBI NIH HHS/United States
GR  - R01 EB002991/EB/NIBIB NIH HHS/United States
GR  - R21 EB014572/EB/NIBIB NIH HHS/United States
GR  - K24HL068796/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120706
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Alexa Fluor 488 C5-maleimide)
RN  - 0 (Drug Carriers)
RN  - 0 (Maleimides)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Polymethacrylic Acids)
RN  - 0 (Receptors, Cell Surface)
RN  - 25087-26-7 (polymethacrylic acid)
RN  - PHA4727WTP (Mannose)
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine/*analogs & derivatives/metabolism
MH  - Administration, Inhalation
MH  - Agglutination/drug effects
MH  - Analysis of Variance
MH  - Animals
MH  - Cells, Cultured
MH  - Drug Carriers/*administration & dosage/chemistry/metabolism
MH  - Macrophages, Alveolar/*metabolism
MH  - Maleimides/chemistry
MH  - Mannose/*analogs & derivatives/metabolism
MH  - Mannose-Binding Lectins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polymethacrylic Acids/administration & dosage/chemical synthesis
MH  - Receptors, Cell Surface/*metabolism
MH  - Spectrophotometry, Ultraviolet
PMC - PMC3449172
MID - NIHMS393362
EDAT- 2012/07/10 06:00
MHDA- 2012/12/10 06:00
PMCR- 2013/10/01
CRDT- 2012/07/10 06:00
PHST- 2012/04/09 00:00 [received]
PHST- 2012/06/16 00:00 [accepted]
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - S0142-9612(12)00661-8 [pii]
AID - 10.1016/j.biomaterials.2012.06.025 [doi]
PST - ppublish
SO  - Biomaterials. 2012 Oct;33(28):6889-97. doi: 10.1016/j.biomaterials.2012.06.025. 
      Epub 2012 Jul 6.