PMID- 22771425
OWN - NLM
STAT- MEDLINE
DCOM- 20130103
LR  - 20120817
IS  - 1943-7811 (Electronic)
IS  - 1525-1578 (Linking)
VI  - 14
IP  - 5
DP  - 2012 Sep
TI  - Diagnosis of BK viral nephropathy in the renal allograft biopsy: role of 
      fluorescence in situ hybridization.
PG  - 494-500
LID - 10.1016/j.jmoldx.2012.04.004 [doi]
AB  - Early recognition of BK viral nephropathy is essential for successful management. 
      Our aim in this study was to evaluate a novel fluorescence in situ hybridization 
      (FISH) assay for detection of BK virus in renal transplant biopsies in the 
      context of standard detection methods. Renal allograft biopsies (n = 108) were 
      analyzed via H&E, immunohistochemistry (IHC) for simian virus 40, and FISH for BK 
      virus. BK virus was detected in 16 (14.8%) cases by H&E, 13 (12%) cases by IHC, 
      18 (16.6%) cases by FISH, and 19 (17.6%) cases by real-time PCR; 24 of 108 showed 
      a discrepancy in >/=1 testing modalities. Comparison of H&E, IHC, and FISH showed 
      no statistical difference in detection of BK virus. However, performing 
      comparisons between the different tissue-based assays in the context of plasma or 
      urine real-time PCR results showed significant improvement in detection of BK by 
      FISH over H&E (P = 0.02) but not IHC (P = 0.07). This novel FISH-based approach 
      for BK virus identification in renal allograft biopsy tissue mirrored real-time 
      PCR results and showed superior performance to detection of inclusions by H&E. 
      Therefore, use of FISH for BK virus detection in the setting of renal allograft 
      biopsy is a useful and sensitive detection method and could be adopted in any 
      laboratory that currently performs FISH analysis.
CI  - Copyright (c) 2012 American Society for Investigative Pathology and the Association 
      for Molecular Pathology. Published by Elsevier Inc. All rights reserved.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Molecular Pathology, Cleveland Clinic, Cleveland, Ohio 44195, USA.
FAU - Portier, Bryce P
AU  - Portier BP
FAU - Hu, Bo
AU  - Hu B
FAU - Chiesa-Vottero, Andres
AU  - Chiesa-Vottero A
FAU - Myles, Jonathan
AU  - Myles J
FAU - Procop, Gary W
AU  - Procop GW
FAU - Tubbs, Raymond R
AU  - Tubbs RR
LA  - eng
PT  - Journal Article
DEP - 20120705
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
SB  - IM
MH  - Adult
MH  - Aged
MH  - BK Virus/genetics/*isolation & purification
MH  - Biopsy
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Kidney/pathology/virology
MH  - Kidney Diseases/*pathology/*virology
MH  - Kidney Transplantation/*pathology
MH  - Male
MH  - Middle Aged
MH  - Polyomavirus Infections/*diagnosis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Transplantation, Homologous
MH  - Tumor Virus Infections/*diagnosis
MH  - Young Adult
EDAT- 2012/07/10 06:00
MHDA- 2013/01/04 06:00
CRDT- 2012/07/10 06:00
PHST- 2011/07/05 00:00 [received]
PHST- 2012/03/29 00:00 [revised]
PHST- 2012/04/05 00:00 [accepted]
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2013/01/04 06:00 [medline]
AID - S1525-1578(12)00139-0 [pii]
AID - 10.1016/j.jmoldx.2012.04.004 [doi]
PST - ppublish
SO  - J Mol Diagn. 2012 Sep;14(5):494-500. doi: 10.1016/j.jmoldx.2012.04.004. Epub 2012 
      Jul 5.