PMID- 22771808
OWN - NLM
STAT- MEDLINE
DCOM- 20121119
LR  - 20120806
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 424
IP  - 3
DP  - 2012 Aug 3
TI  - Evaluation of reference genes for gene expression studies in radish (Raphanus 
      sativus L.) using quantitative real-time PCR.
PG  - 398-403
LID - 10.1016/j.bbrc.2012.06.119 [doi]
AB  - Real-time quantitative reverse transcription PCR (RT-qPCR) is a rapid and 
      reliable method for gene expression studies. Normalization based on reference 
      genes can increase the reliability of this technique; however, recent studies 
      have shown that almost no single reference gene is universal for all possible 
      experimental conditions. In this study, eight frequently used reference genes 
      were investigated, including Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 
      Actin2/7 (ACT), Tubulin alpha-5 (TUA), Tubulin beta-1 (TUB), 18S ribosomal RNA 
      (18SrRNA), RNA polymerase-II transcription factor (RPII), Elongation factor 1-b 
      (EF-1b) and Translation elongation factor 2 (TEF2). Expression stability of 
      candidate reference genes was examined across 27 radish samples, representing a 
      range of tissue types, cultivars, photoperiodic and vernalization treatments, and 
      developmental stages. The eight genes in these sample pools displayed a wide 
      range of Ct values and were variably expressed. Two statistical software 
      packages, geNorm and NormFinder showed that TEF2, RPII and ACT appeared to be 
      relatively stable and therefore the most suitable for use as reference genes. 
      These results facilitate selection of desirable reference genes for accurate gene 
      expression studies in radish.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Xu, Yuanyuan
AU  - Xu Y
AD  - National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key 
      Laboratory of Biology and Genetic Improvement of Horticultural Crops (East 
      China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing 
      Agricultural University, Nanjing 210095, PR China.
FAU - Zhu, Xianwen
AU  - Zhu X
FAU - Gong, Yiqin
AU  - Gong Y
FAU - Xu, Liang
AU  - Xu L
FAU - Wang, Yan
AU  - Wang Y
FAU - Liu, Liwang
AU  - Liu L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120703
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
SB  - IM
MH  - DNA Primers/chemistry/genetics
MH  - *Gene Expression
MH  - *Gene Expression Regulation, Plant
MH  - *Genes, Plant
MH  - Raphanus/*genetics
MH  - Real-Time Polymerase Chain Reaction/*standards
MH  - Reference Standards
EDAT- 2012/07/10 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/07/10 06:00
PHST- 2012/06/18 00:00 [received]
PHST- 2012/06/25 00:00 [accepted]
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - S0006-291X(12)01221-1 [pii]
AID - 10.1016/j.bbrc.2012.06.119 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2012 Aug 3;424(3):398-403. doi: 
      10.1016/j.bbrc.2012.06.119. Epub 2012 Jul 3.