PMID- 22772461
OWN - NLM
STAT- MEDLINE
DCOM- 20121025
LR  - 20120806
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 506
IP  - 1
DP  - 2012 Sep 10
TI  - Increased TERC gene copy number in amniocytes from fetuses with trisomy 18 or a 
      sex chromosome aneuploidy.
PG  - 46-9
LID - 10.1016/j.gene.2012.06.072 [doi]
AB  - OBJECTIVE: Individuals with chromosomal aneuploidies tend to develop 
      malignancies. Telomerase is an enzyme complex that lengthens telomeres and has 
      enhanced expression in numerous malignancies; one of its components is encoded by 
      the TERC gene. In this study, we evaluated the TERC gene copy number in 
      amniocytes from fetuses with aneuploidy, other than trisomy-21. METHODS: In this 
      prospective, basic research study, fluorescence in situ hybridization (FISH) for 
      the TERC gene (3q26) was applied to amniocytes retrieved from 14 fetuses with 
      various aneuploidies and from a control group of 6 fetuses with a normal 
      karyotype, to determine the TERC gene copy number. RESULTS: The percentage of 
      cells with more than two copies of the TERC gene was lowest in the control group 
      (x3=1.2 +/- 0.4%; x4=0 +/- 0%), higher in the sex chromosome aneuploidies (x3=4 +/- 3%; 
      x4=0.7 +/- 0.95%) and even higher in trisomy 18 (x3=10.6 +/- 2.3; x4=4.6 +/- 1.8). The 
      differences were statistically significant (P<0.05). CONCLUSION: The TERC gene 
      copy number is increased in aneuploid amniocytes, which demonstrates their 
      genetic instability and is presumably related to their tendency to develop 
      malignancies.
CI  - Copyright (c) 2012. Published by Elsevier B.V.
FAU - Biron-Shental, Tal
AU  - Biron-Shental T
AD  - Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel.
FAU - Kitay-Cohen, Yona
AU  - Kitay-Cohen Y
FAU - Tene, Tamar
AU  - Tene T
FAU - Sharony, Reuven
AU  - Sharony R
FAU - Amiel, Aliza
AU  - Amiel A
LA  - eng
PT  - Journal Article
DEP - 20120705
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Amniotic Fluid/cytology/metabolism
MH  - *Aneuploidy
MH  - Case-Control Studies
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Female
MH  - Fetus/metabolism
MH  - Gene Dosage
MH  - Genomic Instability
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Prospective Studies
MH  - RNA/*genetics
MH  - *Sex Chromosome Aberrations
MH  - Telomerase/*genetics
MH  - *Trisomy
EDAT- 2012/07/10 06:00
MHDA- 2012/10/26 06:00
CRDT- 2012/07/10 06:00
PHST- 2012/02/29 00:00 [received]
PHST- 2012/06/19 00:00 [revised]
PHST- 2012/06/21 00:00 [accepted]
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2012/10/26 06:00 [medline]
AID - S0378-1119(12)00782-2 [pii]
AID - 10.1016/j.gene.2012.06.072 [doi]
PST - ppublish
SO  - Gene. 2012 Sep 10;506(1):46-9. doi: 10.1016/j.gene.2012.06.072. Epub 2012 Jul 5.