PMID- 22772774
OWN - NLM
STAT- MEDLINE
DCOM- 20150908
LR  - 20220311
IS  - 1618-1255 (Electronic)
IS  - 1618-1247 (Linking)
VI  - 101
IP  - 2
DP  - 2013 Jul
TI  - In vitro analysis of mesenchymal stem cells derived from human teeth and bone 
      marrow.
PG  - 121-32
LID - 10.1007/s10266-012-0075-0 [doi]
AB  - Mesenchymal stem cells derived from human teeth and bone marrow have been 
      characterized by many research groups, but demonstrate inconsistent cellular 
      phenotypes or functions, partly because of differences in culture methodology. 
      Therefore, our aims were to resolve these inconsistencies and discuss the 
      potential uses of these cells in research/clinical applications. We isolated and 
      characterized dental stem cells (DSCs) from the dental pulp, periodontal 
      ligament, apical papilla (APSCs) and dental follicle (DFSCs) of mature and 
      immature teeth, along with bone marrow-derived stem cells (BMSCs) from the iliac 
      crest. We compared the clonogenic and proliferative potentials of these cells in 
      terms of colony-forming efficiency, proliferation potential, population doubling 
      time and cell cycle. All DSCs, particularly APSCs and DFSCs, possessed greater 
      proliferative potential than BMSCs. All stem cells expressed typical mesenchymal 
      and embryonic markers, and developed alizarin red-positive mineralization nodules 
      and Oil red O-positive lipid droplets when cultured in osteogenic and adipogenic 
      media, respectively. Immunocytochemistry revealed that all stem cells developed 
      neuronal markers when cultured in a control medium without neural inductive 
      supplements. After 7 days of neurogenic culture, the differentiated cells showed 
      a transition from fibroblast-like to neuron-like cell bodies with long processes, 
      suggesting that the stem cells differentiated into mature neurons. Karyotyping 
      confirmed that the stem cells maintained a normal karyotype and were 
      chromosomally stable. Our results provide new insights into the physiological 
      properties of stem cells with a normal karyotype and indicate that DSCs are 
      appropriate for basic research and clinical applications.
FAU - Tamaki, Yuichi
AU  - Tamaki Y
AD  - Department of Developmental and Regenerative Dentistry, The Nippon Dental 
      University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 
      102-8159, Japan.
FAU - Nakahara, Taka
AU  - Nakahara T
FAU - Ishikawa, Hiroshi
AU  - Ishikawa H
FAU - Sato, Soh
AU  - Sato S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120707
PL  - Japan
TA  - Odontology
JT  - Odontology
JID - 101134822
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - DNA Primers
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Humans
MH  - In Vitro Techniques
MH  - Karyotyping
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tooth/*chemistry
EDAT- 2012/07/10 06:00
MHDA- 2015/09/09 06:00
CRDT- 2012/07/10 06:00
PHST- 2012/02/20 00:00 [received]
PHST- 2012/05/26 00:00 [accepted]
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2015/09/09 06:00 [medline]
AID - 10.1007/s10266-012-0075-0 [doi]
PST - ppublish
SO  - Odontology. 2013 Jul;101(2):121-32. doi: 10.1007/s10266-012-0075-0. Epub 2012 Jul 
      7.