PMID- 22772872
OWN - NLM
STAT- MEDLINE
DCOM- 20121218
LR  - 20240322
IS  - 1757-9708 (Electronic)
IS  - 1757-9694 (Print)
IS  - 1757-9694 (Linking)
VI  - 4
IP  - 8
DP  - 2012 Aug
TI  - Functional characterization and axonal transport of quantum dot labeled BDNF.
PG  - 953-60
LID - 10.1039/c2ib20062g [doi]
AB  - Brain derived neurotrophic factor (BDNF) plays a key role in the growth, 
      development and maintenance of the central and peripheral nervous systems. 
      Exogenous BDNF activates its membrane receptors at the axon terminal, and 
      subsequently sends regulation signals to the cell body. To understand how a BDNF 
      signal propagates in neurons, it is important to follow the trafficking of BDNF 
      after it is internalized at the axon terminal. Here we labeled BDNF with bright, 
      photostable quantum dots (QD-BDNF) and followed the axonal transport of QD-BDNF 
      in real time in hippocampal neurons. We showed that QD-BDNF was able to bind BDNF 
      receptors and activate downstream signaling pathways. When QD-BDNF was applied to 
      the distal axons of hippocampal neurons, it was observed to be actively 
      transported toward the cell body at an average speed of 1.11 +/- 0.05 mum s(-1). A 
      closer examination revealed that QD-BDNF was transported by both discrete 
      endosomes and multivesicular body-like structures. Our results showed that 
      QD-BDNF could be used to track the movement of exogenous BDNF in neurons over 
      long distances and to study the signaling organelles that contain BDNF.
FAU - Xie, Wenjun
AU  - Xie W
AD  - Department of Chemistry, Stanford University, Stanford, CA, USA.
FAU - Zhang, Kai
AU  - Zhang K
FAU - Cui, Bianxiao
AU  - Cui B
LA  - eng
GR  - K99 NS057906/NS/NINDS NIH HHS/United States
GR  - R00 NS057906/NS/NINDS NIH HHS/United States
GR  - NS057906/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120706
PL  - England
TA  - Integr Biol (Camb)
JT  - Integrative biology : quantitative biosciences from nano to macro
JID - 101478378
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Axonal Transport/*physiology
MH  - Axons/*physiology
MH  - Biotinylation
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - COS Cells
MH  - Cell Membrane/metabolism
MH  - Chlorocebus aethiops
MH  - Chromatography, High Pressure Liquid/methods
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Hippocampus/*metabolism
MH  - Humans
MH  - Microfluidic Analytical Techniques
MH  - Models, Biological
MH  - Nerve Growth Factors/metabolism
MH  - Neurons/*metabolism
MH  - *Quantum Dots
MH  - Receptor, trkB/metabolism
MH  - Recombinant Proteins/metabolism
MH  - Signal Transduction
PMC - PMC3462492
MID - NIHMS393673
EDAT- 2012/07/10 06:00
MHDA- 2012/12/19 06:00
PMCR- 2013/08/23
CRDT- 2012/07/10 06:00
PHST- 2012/07/10 06:00 [entrez]
PHST- 2012/07/10 06:00 [pubmed]
PHST- 2012/12/19 06:00 [medline]
PHST- 2013/08/23 00:00 [pmc-release]
AID - 10.1039/c2ib20062g [doi]
PST - ppublish
SO  - Integr Biol (Camb). 2012 Aug;4(8):953-60. doi: 10.1039/c2ib20062g. Epub 2012 Jul 
      6.