PMID- 22775767
OWN - NLM
STAT- MEDLINE
DCOM- 20121212
LR  - 20140310
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 80
IP  - 3
DP  - 2012 Sep
TI  - An accurate and reliable real time SNP genotyping assay for the HLA-G +3142 bp 
      C>G polymorphism.
PG  - 259-62
LID - 10.1111/j.1399-0039.2012.01926.x [doi]
AB  - Human leukocyte antigen (HLA)-G is a non classical HLA class I antigen with 
      immuno-modulatory functions. The HLA-G gene is characterized by a +3142C>G 
      variant in the 3' untranslated region which is suggested to control protein 
      production and to be associated with pathological conditions. DNAs form 221 
      randomly selected healthy subjects were genotyped for HLA-G +3142C>G polymorphism 
      by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) 
      (BaeGI), real-time PCR and sequencing. The 19% of the PCR-RFLP heterozygous 
      samples were genotyped as 3142GG by real-time PCR and sequencing. This 
      disagreement is caused by digestion efficiency in PCR-RFLP. This real-time PCR 
      method will guarantee an accurate genotyping for future research and clinical 
      purposes, where large cohorts should be tested.
CI  - (c) 2012 John Wiley & Sons A/S.
FAU - Bortolotti, D
AU  - Bortolotti D
AD  - Department of Experimental and Diagnostic Medicine, Section of Microbiology, 
      University of Ferrara, Ferrara, 44121, Italy.
FAU - Gentili, V
AU  - Gentili V
FAU - Melchiorri, L
AU  - Melchiorri L
FAU - Rotola, A
AU  - Rotola A
FAU - Rizzo, R
AU  - Rizzo R
LA  - eng
PT  - Journal Article
DEP - 20120710
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA-G Antigens)
SB  - IM
CIN - Tissue Antigens. 2013 Oct;82(4):286-7. PMID: 24032992
MH  - 3' Untranslated Regions/genetics
MH  - Base Pairing/*genetics
MH  - Base Sequence
MH  - Genotype
MH  - Genotyping Techniques/*methods
MH  - HLA-G Antigens/*genetics
MH  - Health
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymorphism, Restriction Fragment Length/genetics
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
EDAT- 2012/07/11 06:00
MHDA- 2012/12/13 06:00
CRDT- 2012/07/11 06:00
PHST- 2012/02/22 00:00 [received]
PHST- 2012/04/26 00:00 [revised]
PHST- 2012/06/13 00:00 [accepted]
PHST- 2012/07/11 06:00 [entrez]
PHST- 2012/07/11 06:00 [pubmed]
PHST- 2012/12/13 06:00 [medline]
AID - 10.1111/j.1399-0039.2012.01926.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2012 Sep;80(3):259-62. doi: 10.1111/j.1399-0039.2012.01926.x. 
      Epub 2012 Jul 10.