PMID- 22776353
OWN - NLM
STAT- MEDLINE
DCOM- 20121120
LR  - 20131121
IS  - 1365-3156 (Electronic)
IS  - 1360-2276 (Linking)
VI  - 17
IP  - 9
DP  - 2012 Sep
TI  - Rapid identification of Leishmania spp. in formalin-fixed, paraffin-embedded 
      tissue samples by fluorescence in situ hybridization.
PG  - 1117-26
LID - 10.1111/j.1365-3156.2012.03024.x [doi]
AB  - OBJECTIVE: To describe and validate fluorescence in situ hybridization (FISH), a 
      new method of Leishmania spp. identification. FISH allows for a rapid detection 
      of target organisms by specific binding of fluorescently labelled oligonucleotide 
      probes to ribosomal RNA. METHODS: Two genus-specific, fluorescently labelled 
      Leishmania spp. FISH probes were designed and evaluated with a panel of 18 
      Leishmania spp. and six Trypanosoma spp. including well-defined strains and 
      clinical isolates. In addition, the FISH probes were tested in comparison with 
      Giemsa staining in formalin-fixed, paraffin-embedded tissues of five mice that 
      had been artificially infected with Leishmania major strains, leading to 
      concordant results. Finally, 11 tissue samples of patients with cutaneous 
      leishmaniasis, four tissue samples of patients with visceral leishmaniasis, and 
      one native bone marrow sample of a patient with visceral leishmaniasis were 
      analysed with FISH and Giemsa staining. RESULTS: Concordant results were achieved 
      by FISH and Giemsa staining in 15/16 specimens. CONCLUSION: This analysis 
      provides proof of principle that FISH is a suitable method for the rapid and easy 
      detection of Leishmania spp. in formalin-fixed, paraffin-embedded tissue samples. 
      Because of the good contrast of Leishmania spp. in tissue, FISH facilitates the 
      identification of these organisms in tissue samples even by less experienced 
      investigators.
CI  - (c) 2012 Blackwell Publishing Ltd.
FAU - Frickmann, Hagen
AU  - Frickmann H
AD  - Department for Tropical Medicine at the Bernhard Nocht Institute, German Armed 
      Forces Hospital Hamburg, Hamburg, Germany. frickmann@bni-hamburg.de
FAU - Alnamar, Yaser
AU  - Alnamar Y
FAU - Essig, Andreas
AU  - Essig A
FAU - Clos, Joachim
AU  - Clos J
FAU - Racz, Paul
AU  - Racz P
FAU - Barth, Thomas F
AU  - Barth TF
FAU - Hagen, Ralf M
AU  - Hagen RM
FAU - Fischer, Marcellus
AU  - Fischer M
FAU - Poppert, Sven
AU  - Poppert S
LA  - eng
PT  - Journal Article
DEP - 20120709
PL  - England
TA  - Trop Med Int Health
JT  - Tropical medicine & international health : TM & IH
JID - 9610576
RN  - 0 (RNA, Protozoan)
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Animals
MH  - *Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leishmania/*classification
MH  - Leishmaniasis/*diagnosis/parasitology
MH  - Mice
MH  - Paraffin Embedding/*methods
MH  - RNA, Protozoan
MH  - Sequence Analysis, RNA
MH  - Time Factors
MH  - Trypanosoma/classification
EDAT- 2012/07/11 06:00
MHDA- 2012/12/10 06:00
CRDT- 2012/07/11 06:00
PHST- 2012/07/11 06:00 [entrez]
PHST- 2012/07/11 06:00 [pubmed]
PHST- 2012/12/10 06:00 [medline]
AID - 10.1111/j.1365-3156.2012.03024.x [doi]
PST - ppublish
SO  - Trop Med Int Health. 2012 Sep;17(9):1117-26. doi: 
      10.1111/j.1365-3156.2012.03024.x. Epub 2012 Jul 9.