PMID- 22776768
OWN - NLM
STAT- MEDLINE
DCOM- 20130712
LR  - 20161125
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 157
IP  - 2
DP  - 2013 Mar
TI  - Lack of CYP1A responsiveness in species inhabiting chronically contaminated 
      habitats: two varieties of resistance?
PG  - 212-9
LID - S1532-0456(12)00083-X [pii]
LID - 10.1016/j.cbpc.2012.07.001 [doi]
AB  - Organisms chronically exposed to organic pollutants such as polychlorinated 
      biphenyls (PCBs) can develop resistance to these chemicals, a condition 
      associated with reduced inducibility of the biomarker enzyme cytochrome P450 1A 
      (CYP1A). This study addresses the CYP1A response of members of the families 
      Ictaluridae and Centrarchidae, two fish families found throughout much of the 
      United States. We measured CYP1A expression, PCB body burdens, and conducted 
      CYP1A challenge experiments in species from these families residing in the Town 
      Branch/Mud River system (Logan County, KY, USA), a stream system historically 
      contaminated with high levels of PCBs. Despite PCB concentrations in muscle 
      tissue typically associated with elevated CYP1A (16.7 to 75.2mugPCB/g wet edible 
      flesh), resident fish in the contaminated Town Branch/Mud River sites (yellow 
      bullhead [Ameiurus natalis], green sunfish [Lepomis cyanellus], and spotted bass 
      [Micropterus punctulatus]) had hepatic CYP1A activity levels similar to, rather 
      than higher than, those in reference fish, suggesting reduced sensitivity to 
      CYP1A induction. Lack of CYP1A expression following direct contaminant exposure 
      has often been associated with resistance to those contaminants. To determine if 
      CYP1A in resident populations was resistant to induction by PCBs, we exposed 
      resident fish to a single, intraperitoneal injection with a potent CYP1A inducer, 
      3,4,3',4'-tetrachlorobiphenyl (PCB 77). PCB 77 treatment significantly induced 
      hepatic CYP1A activity and protein in yellow bullhead from reference, but not 
      contaminated, sites and had no effect on CYP1A in green sunfish from either site. 
      The low CYP1A expression levels in resident fish with elevated PCB body burdens, 
      together with the failure of PCB injection to induce CYP1A in certain 
      populations, indicate an acclimatory CYP1A response in yellow bullheads and 
      likely an inherently resistant CYP1A in green sunfish. This work demonstrates for 
      the first time acclimation of CYP1A to PCBs in a species within the family 
      Ictaluridae and provides further support for our previous work indicating an 
      apparent inherent lack of CYP1A sensitivity to chlorinated inducers in 
      Centrarchids. These traits may explain, at least in part, the common association 
      of these families with degraded habitats and indicate Lepomis members are likely 
      to be excellent candidates for exploring the mechanistic basis of 'inherent' 
      CYP1A resistance. This study also underlines to the need for thorough 
      characterization of the CYP1A responsivity of a population and/or species prior 
      to using CYP1A as a reliable biomonitoring tool.
CI  - Copyright (c) 2012. Published by Elsevier Inc.
FAU - Brammell, Ben F
AU  - Brammell BF
AD  - Department of Biology, University of Kentucky, Lexington, KY 40506, USA. 
      ben.brammell@asbury.edu
FAU - Price, David J
AU  - Price DJ
FAU - Birge, Wesley J
AU  - Birge WJ
FAU - Elskus, Adria A
AU  - Elskus AA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20120706
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Water Pollutants, Chemical)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - Y2I6546TMI (3,4,3',4'-tetrachlorobiphenyl)
SB  - IM
MH  - Acclimatization/drug effects
MH  - Animals
MH  - Bass/growth & development/metabolism
MH  - Cytochrome P-450 CYP1A1/*metabolism
MH  - Ecosystem
MH  - Environmental Monitoring/methods
MH  - Female
MH  - Geography
MH  - Ictaluridae/growth & development/*metabolism
MH  - Immunoblotting
MH  - Injections, Intraperitoneal
MH  - Kentucky
MH  - Liver/drug effects/enzymology
MH  - Male
MH  - Perciformes/growth & development/*metabolism
MH  - Polychlorinated Biphenyls/administration & dosage/metabolism/*toxicity
MH  - Reproducibility of Results
MH  - Rivers
MH  - Water Pollutants, Chemical/administration & dosage/metabolism/*toxicity
EDAT- 2012/07/11 06:00
MHDA- 2013/07/16 06:00
CRDT- 2012/07/11 06:00
PHST- 2012/01/12 00:00 [received]
PHST- 2012/07/01 00:00 [revised]
PHST- 2012/07/01 00:00 [accepted]
PHST- 2012/07/11 06:00 [entrez]
PHST- 2012/07/11 06:00 [pubmed]
PHST- 2013/07/16 06:00 [medline]
AID - S1532-0456(12)00083-X [pii]
AID - 10.1016/j.cbpc.2012.07.001 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2013 Mar;157(2):212-9. doi: 
      10.1016/j.cbpc.2012.07.001. Epub 2012 Jul 6.