PMID- 22778738
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20120823
LR  - 20230429
IS  - 1687-8779 (Electronic)
IS  - 1687-8760 (Print)
IS  - 1687-8760 (Linking)
VI  - 2012
DP  - 2012
TI  - Live Cells as Dynamic Laboratories: Time Lapse Raman Spectral Microscopy of 
      Nanoparticles with Both IgE Targeting and pH-Sensing Functions.
PG  - 390182
LID - 10.1155/2012/390182 [doi]
LID - 390182
AB  - This review captures the use of live cells as dynamic microlaboratories through 
      implementation of labeled nanoparticles (nanosensors) that have both sensing and 
      targeting functions. The addition of 2,4-epsilon-dinitrophenol-L-lysine (DNP) as a 
      FcepsilonRI targeting ligand and 4-mercaptopyridine (4-MPy) as a pH-sensing ligand 
      enables spatial and temporal monitoring of FcepsilonRI receptors and their pH 
      environment within the endocytic pathway. To ensure reliability, the sensor is 
      calibrated in vivo using the ionophore nigericin and standard buffer solutions to 
      equilibrate the external [H(+)] concentration with that of the cell compartments. 
      This review highlights the nanosensors, ability to traffic and respond to pH of 
      receptor-bound nanosensors (1) at physiological temperature (37 degrees C) versus room 
      temperature (25 degrees C), (2) after pharmacological treatment with bafilomycin, an H(+) 
      ATPase pump inhibitor, or amiloride, an inhibitor of Na(+)/H(+) exchange, and (3) 
      in response to both temperature and pharmacological treatment. Whole-cell, time 
      lapse images are demonstrated to show the ability to transform live cells into 
      dynamic laboratories to monitor temporal and spatial endosomal pH. The 
      versatility of these probes shows promise for future applications relevant to 
      intracellular trafficking and intelligent drug design.
FAU - Nowak-Lovato, Kristy L
AU  - Nowak-Lovato KL
AD  - Decision Applications Division, Los Alamos National Laboratory, Los Alamos, NM 
      87545, USA.
FAU - Rector, Kirk D
AU  - Rector KD
LA  - eng
PT  - Journal Article
DEP - 20120624
PL  - Egypt
TA  - Int J Anal Chem
JT  - International journal of analytical chemistry
JID - 101519424
PMC - PMC3388588
EDAT- 2012/07/11 06:00
MHDA- 2012/07/11 06:01
PMCR- 2012/06/24
CRDT- 2012/07/11 06:00
PHST- 2012/03/01 00:00 [received]
PHST- 2012/05/01 00:00 [accepted]
PHST- 2012/07/11 06:00 [entrez]
PHST- 2012/07/11 06:00 [pubmed]
PHST- 2012/07/11 06:01 [medline]
PHST- 2012/06/24 00:00 [pmc-release]
AID - 10.1155/2012/390182 [doi]
PST - ppublish
SO  - Int J Anal Chem. 2012;2012:390182. doi: 10.1155/2012/390182. Epub 2012 Jun 24.